Acetaldehyde disrupts tight junctions and adherens junctions in human colonic mucosa

Protection by EGF and L-glutamine

S. Basuroy, P. Sheth, C. M. Mansbach, Radhakrishna Rao

Research output: Contribution to journalArticle

87 Citations (Scopus)

Abstract

Acetaldehyde, a toxic metabolite of ethanol oxidation, is suggested to play a role in the increased risk for gastrointestinal cancers in alcoholics. In the present study, the effect of acetaldehyde on tyrosine phosphorylation, immmunofluorescence localization, and detergent-insoluble fractions of the tight junction and the adherens junction proteins was determined in the human colonic mucosa. The role of EGF and L-glutamine in prevention of acetaldehyde-induced effects was also evaluated. Acetaldehyde reduced the protein tyrosine phosphatase activity, thereby increasing the tyrosine phosphorylation of occludin, E-cadherin, and β-catenin. The levels of occludin, zonula occludens-1, E-cadherin, and β-catenin in detergent-insoluble fractions were reduced by acetaldehyde, while it increased their levels in detergent-soluble fractions. Pretreatment with EGF or L-glutamine prevented acetaldehyde-induced protein tyrosine phosphorylation, redistribution from intercellular junctions, and reduction in the levels of detergent-insoluble fractions of occludin, zonula occludens-1, E-cadherin, and β-catenin. These results demonstrate that acetaldehyde induces tyrosine phosphorylation and disrupts tight junction and adherens junction in human colonic mucosa, which can be prevented by EGF and glutamine.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Gastrointestinal and Liver Physiology
Volume289
Issue number2 52-2
DOIs
StatePublished - Aug 1 2005

Fingerprint

Adherens Junctions
Acetaldehyde
Tight Junctions
Glutamine
Epidermal Growth Factor
Mucous Membrane
Occludin
Catenins
Detergents
Tyrosine
Cadherins
Phosphorylation
Protein Tyrosine Phosphatases
Gastrointestinal Neoplasms
Intercellular Junctions
Poisons
Alcoholics
Proteins
Ethanol

All Science Journal Classification (ASJC) codes

  • Physiology
  • Hepatology
  • Gastroenterology
  • Physiology (medical)

Cite this

@article{58894a872c4a49d98e9f9780c7f787c0,
title = "Acetaldehyde disrupts tight junctions and adherens junctions in human colonic mucosa: Protection by EGF and L-glutamine",
abstract = "Acetaldehyde, a toxic metabolite of ethanol oxidation, is suggested to play a role in the increased risk for gastrointestinal cancers in alcoholics. In the present study, the effect of acetaldehyde on tyrosine phosphorylation, immmunofluorescence localization, and detergent-insoluble fractions of the tight junction and the adherens junction proteins was determined in the human colonic mucosa. The role of EGF and L-glutamine in prevention of acetaldehyde-induced effects was also evaluated. Acetaldehyde reduced the protein tyrosine phosphatase activity, thereby increasing the tyrosine phosphorylation of occludin, E-cadherin, and β-catenin. The levels of occludin, zonula occludens-1, E-cadherin, and β-catenin in detergent-insoluble fractions were reduced by acetaldehyde, while it increased their levels in detergent-soluble fractions. Pretreatment with EGF or L-glutamine prevented acetaldehyde-induced protein tyrosine phosphorylation, redistribution from intercellular junctions, and reduction in the levels of detergent-insoluble fractions of occludin, zonula occludens-1, E-cadherin, and β-catenin. These results demonstrate that acetaldehyde induces tyrosine phosphorylation and disrupts tight junction and adherens junction in human colonic mucosa, which can be prevented by EGF and glutamine.",
author = "S. Basuroy and P. Sheth and Mansbach, {C. M.} and Radhakrishna Rao",
year = "2005",
month = "8",
day = "1",
doi = "10.1152/ajpgi.00464.2004",
language = "English (US)",
volume = "289",
journal = "American Journal of Physiology",
issn = "1931-857X",
publisher = "American Physiological Society",
number = "2 52-2",

}

TY - JOUR

T1 - Acetaldehyde disrupts tight junctions and adherens junctions in human colonic mucosa

T2 - Protection by EGF and L-glutamine

AU - Basuroy, S.

AU - Sheth, P.

AU - Mansbach, C. M.

AU - Rao, Radhakrishna

PY - 2005/8/1

Y1 - 2005/8/1

N2 - Acetaldehyde, a toxic metabolite of ethanol oxidation, is suggested to play a role in the increased risk for gastrointestinal cancers in alcoholics. In the present study, the effect of acetaldehyde on tyrosine phosphorylation, immmunofluorescence localization, and detergent-insoluble fractions of the tight junction and the adherens junction proteins was determined in the human colonic mucosa. The role of EGF and L-glutamine in prevention of acetaldehyde-induced effects was also evaluated. Acetaldehyde reduced the protein tyrosine phosphatase activity, thereby increasing the tyrosine phosphorylation of occludin, E-cadherin, and β-catenin. The levels of occludin, zonula occludens-1, E-cadherin, and β-catenin in detergent-insoluble fractions were reduced by acetaldehyde, while it increased their levels in detergent-soluble fractions. Pretreatment with EGF or L-glutamine prevented acetaldehyde-induced protein tyrosine phosphorylation, redistribution from intercellular junctions, and reduction in the levels of detergent-insoluble fractions of occludin, zonula occludens-1, E-cadherin, and β-catenin. These results demonstrate that acetaldehyde induces tyrosine phosphorylation and disrupts tight junction and adherens junction in human colonic mucosa, which can be prevented by EGF and glutamine.

AB - Acetaldehyde, a toxic metabolite of ethanol oxidation, is suggested to play a role in the increased risk for gastrointestinal cancers in alcoholics. In the present study, the effect of acetaldehyde on tyrosine phosphorylation, immmunofluorescence localization, and detergent-insoluble fractions of the tight junction and the adherens junction proteins was determined in the human colonic mucosa. The role of EGF and L-glutamine in prevention of acetaldehyde-induced effects was also evaluated. Acetaldehyde reduced the protein tyrosine phosphatase activity, thereby increasing the tyrosine phosphorylation of occludin, E-cadherin, and β-catenin. The levels of occludin, zonula occludens-1, E-cadherin, and β-catenin in detergent-insoluble fractions were reduced by acetaldehyde, while it increased their levels in detergent-soluble fractions. Pretreatment with EGF or L-glutamine prevented acetaldehyde-induced protein tyrosine phosphorylation, redistribution from intercellular junctions, and reduction in the levels of detergent-insoluble fractions of occludin, zonula occludens-1, E-cadherin, and β-catenin. These results demonstrate that acetaldehyde induces tyrosine phosphorylation and disrupts tight junction and adherens junction in human colonic mucosa, which can be prevented by EGF and glutamine.

UR - http://www.scopus.com/inward/record.url?scp=22544451542&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=22544451542&partnerID=8YFLogxK

U2 - 10.1152/ajpgi.00464.2004

DO - 10.1152/ajpgi.00464.2004

M3 - Article

VL - 289

JO - American Journal of Physiology

JF - American Journal of Physiology

SN - 1931-857X

IS - 2 52-2

ER -