Acetylcholine dramatically increases prostanoid synthesis in piglet parietal cortex

David W. Busija, L. Craig Wagerle, Massroor Pourcyrous, Charles Leffler

Research output: Contribution to journalArticle

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Abstract

We investigated effects of exogenous acetylcholine on prostanoid synthesis by parietal cortex in neonatal pigs. Cerebrospinal fluid (CSF) with no drug, and CSF containing acetylcholine at 10 -6 to 10 -3 M was injected under a 'closed' cranial window, and after 5 min the CSF was collected and analyzed by radioimmunoassay for prostaglandin (PG) E 2 , PGF , PGD 2 , 6-keto-PGF (the hydrolysis product of prostacyclin), and thromboxane (TX) B 2 (the hydrolysis product of TXA 2 ). PGE 2 and PGF were the predominant prostanoids in CSF under control conditions. Levels of all CSF prostanoids increased after topical application of acetylcholine, with the largest increases being for PGE 2 and PGF . During control conditions, levels were 1294 ± 170 (mean ± S.E.M.) pg/ml for PGE 2 (n = 16). 1032 ± 143 pg/ml for PGF (n = 3), 659 ± 92 pg/ml for 6-keto-PGF (n = 15), 141 ± 44 pg/ml for TXB 2 (n = 12), and were below detectable levels for PGD 2 . Following application of 10 -3 M acetylcholine, levels were 34,535 ± 5438 pg/ml for PGE 2 , 15,539 ± 2772 pg/ml for PGF , 2967 ± 547 pg/ml for 6-keto-PGF , 580 ± 105 pg/ml for TXB 2 , and 556 ± 221 pg/ml for PGD 2 These results suggest that prostanoids could play a role in mediating effects of acetylcholine in the brain, or in modulating acetylcholine release via a negative feedback mechanism.

Original languageEnglish (US)
Pages (from-to)122-126
Number of pages5
JournalBrain Research
Volume439
Issue number1-2
DOIs
StatePublished - Jan 26 1988

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Parietal Lobe
Prostaglandins F
Acetylcholine
Prostaglandins
Prostaglandins E
Cerebrospinal Fluid
Prostaglandins D
Hydrolysis
Thromboxanes
Epoprostenol
Radioimmunoassay
Swine
Brain

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

Cite this

Acetylcholine dramatically increases prostanoid synthesis in piglet parietal cortex. / Busija, David W.; Craig Wagerle, L.; Pourcyrous, Massroor; Leffler, Charles.

In: Brain Research, Vol. 439, No. 1-2, 26.01.1988, p. 122-126.

Research output: Contribution to journalArticle

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