Acetylcholine release in the pontine reticular formation of C57BL/6J mouse is modulated by non-M1 muscarinic receptors

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Abstract

Pontine acetylcholine (ACh) contributes to the regulation of electroencephalographic and behavioral arousal in all mammals so far investigated. The mouse is recognized as a powerful model for pharmacogenomics but the synaptic mechanisms regulating ACh release in mouse pontine reticular formation have not been characterized. Drug delivery by microdialysis was used in isoflurane-anesthetized C57BL/6J (B6) mice (n=33) to test the hypothesis that muscarinic autoreceptors modulate ACh release in the pontine reticular nucleus, oral part (PnO). Dialysis delivery of tetrodotoxin to the PnO significantly decreased ACh by 58% below control levels, confirming that measured ACh reflected neurotransmitter release. The muscarinic antagonist scopolamine increased ACh release in the PnO by 21% (3 nM), 48% (10 nM), 56% (30 nM), and 104% (100 nM). The muscarinic agonist bethanechol dialyzed into the PnO significantly decreased ACh release by 60% compared with control. Dialysis delivery of relatively subtype selective muscarinic antagonists to the PnO revealed the following order of potency for increasing ACh release: scopolamine (3 nM)>AF-DX 116 (100 nM)=pirenzepine (100 nM). These data support the conclusion that ACh release in PnO of B6 mouse is modulated by non-M1 muscarinic receptors.

Original languageEnglish (US)
Pages (from-to)831-838
Number of pages8
JournalNeuroscience
Volume126
Issue number4
DOIs
StatePublished - Jun 28 2004
Externally publishedYes

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Muscarinic Receptors
Inbred C57BL Mouse
Acetylcholine
Muscarinic Antagonists
Scopolamine Hydrobromide
Dialysis
Bethanechol
Pirenzepine
Pontine Tegmentum
Muscarinic Agonists
Autoreceptors
Pharmacogenetics
Isoflurane
Microdialysis
Tetrodotoxin
Arousal
Cholinergic Agents
Neurotransmitter Agents
Mammals

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

Acetylcholine release in the pontine reticular formation of C57BL/6J mouse is modulated by non-M1 muscarinic receptors. / Coleman, C. G.; Lydic, Ralph; Baghdoyan, Helen.

In: Neuroscience, Vol. 126, No. 4, 28.06.2004, p. 831-838.

Research output: Contribution to journalArticle

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