Acetylcholinesterase and Nissl staining in the same histological section

M. T. Shipley, Matthew Ennis, M. M. Behbehani

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Acetylcholinesterase (AChE) enzyme histochemistry and Nissl staining are commonly utilized in neural architectonic studies. However, the opaque reaction deposit produced by the most commonly used AChE histochemical methods is not compatible with satisfactory Nissl staining. As a result, precise correlation of AChE and Nissl staining necessitates time-consuming comparisons of adjacent sections which may have differential shrinkage. Here, we have modified the Koelle-Friedenwald histochemical reaction for AChE by omitting the final intensification steps. The modified reaction yields a non-opaque reaction product that is selectively visualized by darkfield illumination. This non-intensified darkfield AChE (NIDA) reaction allows clear visualization of Nissl staining in the same histological section. This combined AChE-Nissl method greatly facilitates detailed correlation of enzyme and cytoarchitectonic organization.

Original languageEnglish (US)
Pages (from-to)347-353
Number of pages7
JournalBrain Research
Volume504
Issue number2
DOIs
StatePublished - Dec 18 1989
Externally publishedYes

Fingerprint

Acetylcholinesterase
Staining and Labeling
Enzymes
Lighting

All Science Journal Classification (ASJC) codes

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

Cite this

Acetylcholinesterase and Nissl staining in the same histological section. / Shipley, M. T.; Ennis, Matthew; Behbehani, M. M.

In: Brain Research, Vol. 504, No. 2, 18.12.1989, p. 347-353.

Research output: Contribution to journalArticle

Shipley, M. T. ; Ennis, Matthew ; Behbehani, M. M. / Acetylcholinesterase and Nissl staining in the same histological section. In: Brain Research. 1989 ; Vol. 504, No. 2. pp. 347-353.
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