Acidic and basic fibroblast growth factors involved in cardiac angiogenesis following infarction

Tieqiang Zhao, Wenyuan Zhao, Yuanjian Chen, Robert A. Ahokas, Yao Sun

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Acidic and basic fibroblast growth factors (FGF-1/FGF-2) promote angiogenesis in cancer. Angiogenesis is integral to cardiac repair following myocardial infarction (MI). The potential regulation of FGF-1/FGF-2 in cardiac angiogenesis postMI remains unexplored. Herein, we examined the temporal and spatial expression of FGF-1/FGF-2 and FGF receptors (FGFR) in the infarcted rat heart at days 1, 3, 7, and 14 postMI. FGF-1/-2 gene and protein expression, cells expressing FGF-1/-2 and FGFR expression were examined by quantitative in situ hybridization, RT-PCR; western blot, immunohistochemistry and quantitative in vitro autoradiography. Compared to the normal heart, we found that in the border zone and infarcted myocardium 1) FGF-1 gene expression was increased in the first week postMI and returned to control levels at week 2; FGF-1 protein levels were, however, largely reduced at day 1, then elevated at day 3 peaked at day 7 and declined at day 14; and cells expressing FGF-1 were primarily inflammatory cells; 2) FGF-2 gene expression was significantly elevated from day 1 to day 14; the increase in FGF-2 protein level was most evident at day 7 and cells expressing FGF-2 were primarily endothelial cells; 3) FGFR expression started to increase at day 3 and remained elevated thereafter; and 4) FGF-1/FGF-2 and FGFR expression remained unchanged in the noninfarcted myocardium. Thus, FGF-1/FGF-2 and FGFR expression are enhanced in the infarcted myocardium in the early stage after MI, which is spatially and temporally coincident with angiogenesis, suggesting that FGF-1/FGF-2 are involved in regulating cardiac angiogenesis and repair.

Original languageEnglish (US)
Pages (from-to)307-313
Number of pages7
JournalInternational Journal of Cardiology
Volume152
Issue number3
DOIs
StatePublished - Nov 3 2011

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Fibroblast Growth Factor 1
Fibroblast Growth Factor 2
Infarction
Fibroblast Growth Factor Receptors
Myocardium
Gene Expression
Myocardial Infarction
Proteins
Autoradiography
In Situ Hybridization

All Science Journal Classification (ASJC) codes

  • Medicine(all)
  • Cardiology and Cardiovascular Medicine

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Acidic and basic fibroblast growth factors involved in cardiac angiogenesis following infarction. / Zhao, Tieqiang; Zhao, Wenyuan; Chen, Yuanjian; Ahokas, Robert A.; Sun, Yao.

In: International Journal of Cardiology, Vol. 152, No. 3, 03.11.2011, p. 307-313.

Research output: Contribution to journalArticle

Zhao, Tieqiang ; Zhao, Wenyuan ; Chen, Yuanjian ; Ahokas, Robert A. ; Sun, Yao. / Acidic and basic fibroblast growth factors involved in cardiac angiogenesis following infarction. In: International Journal of Cardiology. 2011 ; Vol. 152, No. 3. pp. 307-313.
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