Actinomycin D blocks interleukin-1α-induced pial arteriolar dilation and increased prostanoid production in newborn pigs

Masaaki Shibata, Robert Mirro, William M. Armstead, Charles Leffler

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Effects of protein synthesis and cyclooxygenase inhibitors on interleukin-1α (IL-1α)- and histamine-induced pial arteriolar dilation and cerebrospinal fluid (CSF) prostanoid increases were examined in anesthetized piglets using closed cranial windows. Topical IL-la (10.8 μg) increased pial arteriolar diameter from 15 to 30 min after its infusion, and enhanced CSF prostanoids. Topical protein synthesis inhibitor, actinomycin D, at a concentration of 10-8 M attenuated and 10-8 M completely blocked both IL-1α-induced vasodilation and CSF prostanoid increase. Inhibition of prostaglandin H synthases with indomethacin blocked both vasodilation and CSF prostanoid increase by IL-1α. Topical histamine (10-6 M) also increased pial arteriolar diameter and CSF prostanoids but without the delay seen between IL-1α infusion and responses. These histamine effects were not modified by coinfusion of actinomycin D but blocked by indomethacin. These results suggest that, although IL-1α and histamine do share the same mechanism insofar as activation of prostaglandin synthesis is concerned, an additional step appears to be involved for IL-1α, likely involving de novo protein synthesis.

Original languageEnglish (US)
Pages (from-to)403-409
Number of pages7
JournalBrain Research Bulletin
Volume33
Issue number4
DOIs
StatePublished - Jan 1 1994

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Dactinomycin
Interleukin-1
Prostaglandins
Dilatation
Swine
Cerebrospinal Fluid
Histamine
Protein Synthesis Inhibitors
Vasodilation
Indomethacin
Histamine Agents
Cyclooxygenase Inhibitors
Prostaglandin-Endoperoxide Synthases
Proteins

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

Actinomycin D blocks interleukin-1α-induced pial arteriolar dilation and increased prostanoid production in newborn pigs. / Shibata, Masaaki; Mirro, Robert; Armstead, William M.; Leffler, Charles.

In: Brain Research Bulletin, Vol. 33, No. 4, 01.01.1994, p. 403-409.

Research output: Contribution to journalArticle

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