Activation of diacylglycerol in cultured endothelial cells exposed to cyclic strain

Leigh Evans, Lijubov Frenkel, Colleen M. Brophy, Oscar Rosales, Chittoor Sai Sudhakar, Guangdi Li, Wei Du, Bauer E. Sumpio

Research output: Contribution to journalArticle

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Abstract

Confluent bovine aortic endothelial cells (EC) were grown on flexible membranes and subjected to 10% average strain at 60 cycles/min for up to 500 s. A biphasic increase in diacylglycerol (DAG) occurred, with an initial transient peak at 10 s followed by sustained elevation to 500 s. The early peak corresponded to the transient formation of inositol 1,4,5-trisphosphate, demonstrating hydrolysis of L-α-phosphatidylinositol (PI) by PI-specific phospholipase C. To determine the origin of the sustained DAG phase, we incubated confluent bovine aortic EC with 1 μCi/ml [14C]myristate overnight and subjected them to cyclic strain. There was a decrease in phosphatidylcholine (PC) and a corresponding increase in DAG at 10 s and 250 s, suggesting PC hydrolysis with the generation of DAG at both an early (10 s) and a late (250 s) phase. [14C]phosphatidylethanol, a specific product of phospholipase D (PLD) in the presence of 1% ethanol, was measured in EC preincubated with [14C]myristate. Cyclic strain led to an immediate and sustained activation of PLD. Increased ethanol concentration led to a consistent decrease in DAG. Furthermore, when EC were pretreated with 1% ethanol, the strain-induced proliferative response was attenuated.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Cell Physiology
Volume272
Issue number2 41-2
StatePublished - Feb 1 1997
Externally publishedYes

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Diglycerides
Cultured Cells
Endothelial Cells
Phospholipase D
Ethanol
Myristic Acid
Phosphatidylcholines
Hydrolysis
Phosphoinositide Phospholipase C
Inositol 1,4,5-Trisphosphate
Phosphatidylinositols
Membranes

All Science Journal Classification (ASJC) codes

  • Physiology
  • Cell Biology

Cite this

Evans, L., Frenkel, L., Brophy, C. M., Rosales, O., Sai Sudhakar, C., Li, G., ... Sumpio, B. E. (1997). Activation of diacylglycerol in cultured endothelial cells exposed to cyclic strain. American Journal of Physiology - Cell Physiology, 272(2 41-2).

Activation of diacylglycerol in cultured endothelial cells exposed to cyclic strain. / Evans, Leigh; Frenkel, Lijubov; Brophy, Colleen M.; Rosales, Oscar; Sai Sudhakar, Chittoor; Li, Guangdi; Du, Wei; Sumpio, Bauer E.

In: American Journal of Physiology - Cell Physiology, Vol. 272, No. 2 41-2, 01.02.1997.

Research output: Contribution to journalArticle

Evans, L, Frenkel, L, Brophy, CM, Rosales, O, Sai Sudhakar, C, Li, G, Du, W & Sumpio, BE 1997, 'Activation of diacylglycerol in cultured endothelial cells exposed to cyclic strain', American Journal of Physiology - Cell Physiology, vol. 272, no. 2 41-2.
Evans, Leigh ; Frenkel, Lijubov ; Brophy, Colleen M. ; Rosales, Oscar ; Sai Sudhakar, Chittoor ; Li, Guangdi ; Du, Wei ; Sumpio, Bauer E. / Activation of diacylglycerol in cultured endothelial cells exposed to cyclic strain. In: American Journal of Physiology - Cell Physiology. 1997 ; Vol. 272, No. 2 41-2.
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