Activation of nuclear factor-κB and its proinflammatory mediator cascade in the infarcted rat heart

Li Lu, Sue S. Chen, John Q. Zhang, Felix J. Ramires, Yao Sun

Research output: Contribution to journalArticle

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Abstract

Nuclear transcription factor (NF)-κB regulates inflammatory and immune responses by increasing the expression of specific inflammatory genes in various tissues. Whether the infarcted heart includes the activation of NF-κB and a proinflammatory mediator cascade that it regulates has not been fully explored. Herein, we monitored the temporal and spatial activation of NF-κB, together with expression of tumor necrosis factor (TNF)-α, vascular cell adhesion molecule-1 (VCAM-1), and transforming growth factor (TGF)-β 1, in the infarcted rat heart at and remote to MI from day 3 to day 28 following left coronary artery ligation. Compared to the normal heart, we observed NF-κB activation, together with the elevated expression of VCAM-1 in endothelial cells, TNF-α and TGF-β 1 in inflammatory cells at sites of repair in the infarcted heart, which started on day 3, peaked on day 7, and gradually declined thereafter. Our findings suggest NF-κB activation and its proinflammatory mediator cascade are contributory to the inflammatory response and remodeling that appear at various sites of repair in the infarcted rat heart.

Original languageEnglish (US)
Pages (from-to)879-885
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume321
Issue number4
DOIs
StatePublished - Sep 3 2004

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Rats
Transcription Factors
Chemical activation
Vascular Cell Adhesion Molecule-1
Transforming Growth Factors
Repair
Tumor Necrosis Factor-alpha
Endothelial cells
Ligation
Coronary Vessels
Endothelial Cells
Genes
Tissue

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Activation of nuclear factor-κB and its proinflammatory mediator cascade in the infarcted rat heart. / Lu, Li; Chen, Sue S.; Zhang, John Q.; Ramires, Felix J.; Sun, Yao.

In: Biochemical and Biophysical Research Communications, Vol. 321, No. 4, 03.09.2004, p. 879-885.

Research output: Contribution to journalArticle

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