Activation of oxide synl'hetase by insulin and possiref "messenger" r01.k n f no in insulin kh-t.cts in various tissues

K. Sinha, K. Acharya, Sujoy Bhattacharya, R. 'Xcharya, S. Mazumder

Research output: Contribution to journalArticle

Abstract

The ident i tv of the "messenger" of insulin mediated effects in Insulin responsive tissue? remains elusive. Herein we report that the exposure of various tissues from heart, liver, Intestine, Kidney, and skeletal muscle} of mice to L50-200 jjU/nl insulin at 'i7£lC for 1 to J h in Kreb's bufter resu11 ed in 4 tu 10 fold increase of NO in the medium over the control, depending on tissue sources, as determined bv spectropho tome trie assay usine, Oxyhemolnbin. Similarly, treatment of homoe,enates of these tissues with insulin resulted in stimulated NO svnthesis in assay mixture in a dose dopenden t manner. Kor the optimal insulin effect, presence of both arin Lne and glucose are needed in the mixture. Further, incubation of these tissues with NO, like insulin itself, resulted in increasd e, lue ose and ami no acid uptake in all cases. These results su,;est that NO miht be the "messenger" of insulin, at least parti v in some tissues, and the hormone probably mediates its effect1-; through the activation of NO- vnthe tasc.

Original languageEnglish (US)
JournalFASEB Journal
Volume11
Issue number9
StatePublished - Dec 1 1997
Externally publishedYes

Fingerprint

Oxides
Chemical activation
Insulin
Tissue
Assays
Liver
Intestines
Muscle
Skeletal Muscle
Hormones
Kidney
Glucose
Acids

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

Cite this

Activation of oxide synl'hetase by insulin and possiref "messenger" r01.k n f no in insulin kh-t.cts in various tissues. / Sinha, K.; Acharya, K.; Bhattacharya, Sujoy; 'Xcharya, R.; Mazumder, S.

In: FASEB Journal, Vol. 11, No. 9, 01.12.1997.

Research output: Contribution to journalArticle

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