Activin A expression regulates multipotency of mesenchymal progenitor cells

Farida Djouad, Wesley M. Jackson, Brent E. Bobick, Sasa Janjanin, Yingjie Song, George Huang, Rocky S. Tuan

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Introduction. Bone marrow (BM) stroma currently represents the most common and investigated source of mesenchymal progenitor cells (MPCs); however, comparable adult progenitor or stem cells have also been isolated from a wide variety of tissues. This study aims to assess the functional similarities of MPCs from different tissues and to identify specific factor(s) related to their multipotency. Methods. For this purpose, we directly compared MPCs isolated from different adult tissues, including bone marrow, tonsil, muscle, and dental pulp. We first examined and compared proliferation rates, immunomodulatory properties, and multidifferentiation potential of these MPCs in vitro. Next, we specifically evaluated activin A expression profile and activin A:follistatin ratio in MPCs from the four sources. Results: The multidifferentiation potential of the MPCs is correlated with activin A level and/or the activin A:follistatin ratio. Interestingly, by siRNA-mediated activin A knockdown, activin A was shown to be required for the chondrogenic and osteogenic differentiation of MPCs. These findings strongly suggest that activin A has a pivotal differentiation-related role in the early stages of chondrogenesis and osteogenesis while inhibiting adipogenesis of MPCs. Conclusions: This comparative analysis of MPCs from different tissue sources also identifies bone marrow-derived MPCs as the most potent MPCs in terms of multilineage differentiation and immunosuppression, two key requirements in cell-based regenerative medicine. In addition, this study implicates the significance of activin A as a functional marker of MPC identity.

Original languageEnglish (US)
Article number11
JournalStem Cell Research and Therapy
Volume1
Issue number2
DOIs
StatePublished - Dec 1 2010

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Mesenchymal Stromal Cells
Follistatin
Tissue
Bone
Bone Marrow
activin A
Stem Cells
Stem cells
Small Interfering RNA
Pulp
Muscle
Chondrogenesis
Dental Pulp
Adipogenesis
Regenerative Medicine
Palatine Tonsil
Osteogenesis
Immunosuppression
Muscles

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology (miscellaneous)
  • Molecular Medicine
  • Cell Biology
  • Medicine (miscellaneous)

Cite this

Djouad, F., Jackson, W. M., Bobick, B. E., Janjanin, S., Song, Y., Huang, G., & Tuan, R. S. (2010). Activin A expression regulates multipotency of mesenchymal progenitor cells. Stem Cell Research and Therapy, 1(2), [11]. https://doi.org/10.1186/scrt11

Activin A expression regulates multipotency of mesenchymal progenitor cells. / Djouad, Farida; Jackson, Wesley M.; Bobick, Brent E.; Janjanin, Sasa; Song, Yingjie; Huang, George; Tuan, Rocky S.

In: Stem Cell Research and Therapy, Vol. 1, No. 2, 11, 01.12.2010.

Research output: Contribution to journalArticle

Djouad, F, Jackson, WM, Bobick, BE, Janjanin, S, Song, Y, Huang, G & Tuan, RS 2010, 'Activin A expression regulates multipotency of mesenchymal progenitor cells', Stem Cell Research and Therapy, vol. 1, no. 2, 11. https://doi.org/10.1186/scrt11
Djouad, Farida ; Jackson, Wesley M. ; Bobick, Brent E. ; Janjanin, Sasa ; Song, Yingjie ; Huang, George ; Tuan, Rocky S. / Activin A expression regulates multipotency of mesenchymal progenitor cells. In: Stem Cell Research and Therapy. 2010 ; Vol. 1, No. 2.
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