Activity level, apoptosis, and development of cachexia in Apc Min/+ mice

Kristen A. Baltgalvis, Franklin G. Berger, Maria Marjorette O. Peña, J. Mark Davis, James P. White, James Carson

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Criteria for diagnosing cachexia in adults include unintentional loss in body weight, decreased strength, fatigue, anorexia, and low muscle mass.Cachexia is also associated with systemic inflammation, altered metabolism,and anemia. The ApcMin/+ mouse is a model of cachexia directly related to intestinal tumor burden and subsequent chronic inflammation. These mice also demonstrate muscle weakness, fatigue, decreased volitional activity, and elevated circulating IL-6 levels. The purpose of this study was to determine the time course of changes in physical activity and their relationship to anemia, muscle apoptosis, and muscle mass and body mass loss during cachexia. A subset of male ApcMin/+ mice were given access to voluntary activity wheels from 5 to 26 wk of age, while sedentary male ApcMin/+ mice were housed in cages lacking wheels. At the study's end mice were stratified by cachectic symptoms. Severely cachectic mice haddecreased wheel running performance at 15 wk of age, while anemia and body weight loss were not present until 18 wk of age. Severely cachectic mice had lower hemoglobin levels compared with mildly cachectic mice at 13, 18, and 22 wk of age. Severely cachectic mice also demonstrated threefold more BCL2-associated X protein (BAX) protein in the gastrocnemius muscle at 26 wk of age compared with mildly cachectic mice. In sedentary ApcMin/+ mice at 26 wk of age anemia was present, and markers of apoptosis were induced in severely cachectic muscle. Proapoptotic protein expression was induced in both red and white portions of gastrocnemius muscle as well as in soleus muscle of severely cachectic mice compared with mildly cachectic mice. These data demonstrate that decrements in wheel running performance precede loss of body mass and that inherent muscle oxidative capacity is not protective against muscle apoptosis.

Original languageEnglish (US)
Pages (from-to)1155-1161
Number of pages7
JournalJournal of applied physiology
Volume109
Issue number4
DOIs
StatePublished - Oct 1 2010

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Cachexia
Apoptosis
Muscles
Anemia
Skeletal Muscle
Running
Body Weight
Inflammation
Muscle Fatigue
Proteins
Muscle Weakness
Anorexia
Tumor Burden
Fatigue
Weight Loss
Interleukin-6
Hemoglobins

All Science Journal Classification (ASJC) codes

  • Physiology
  • Physiology (medical)

Cite this

Baltgalvis, K. A., Berger, F. G., Peña, M. M. O., Davis, J. M., White, J. P., & Carson, J. (2010). Activity level, apoptosis, and development of cachexia in Apc Min/+ mice. Journal of applied physiology, 109(4), 1155-1161. https://doi.org/10.1152/japplphysiol.00442.2010

Activity level, apoptosis, and development of cachexia in Apc Min/+ mice. / Baltgalvis, Kristen A.; Berger, Franklin G.; Peña, Maria Marjorette O.; Davis, J. Mark; White, James P.; Carson, James.

In: Journal of applied physiology, Vol. 109, No. 4, 01.10.2010, p. 1155-1161.

Research output: Contribution to journalArticle

Baltgalvis, Kristen A. ; Berger, Franklin G. ; Peña, Maria Marjorette O. ; Davis, J. Mark ; White, James P. ; Carson, James. / Activity level, apoptosis, and development of cachexia in Apc Min/+ mice. In: Journal of applied physiology. 2010 ; Vol. 109, No. 4. pp. 1155-1161.
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