Adipocyte differentiation stably induces C9 complement- related antiviral activity

R. E. Scott, C. Y. Tzen, S. N. Constantinescu, Lawrence Pfeffer

Research output: Contribution to journalArticle

Abstract

One of the multiple steps in the process of adipocyte differentiation in 3T3T mesenchymal stem cells and their derivatives is designated nonterminal differentiation. At this state, adipocytes can be induced to reenter the cell cycle and proliferate and in doing so lose their differentiated phenotype. Nonterminally differentiated adipocytes and cells derived from them can stably express distinct characteristics, including resistance to neoplastic transformation. The current studies were performed to determine if another of these characteristics includes the expression of interferon-associated antiviral activity. The results establish that differentiation does induce the expression of antiviral activity in adipocyte-conditioned media; however, a variety of immunological and molecular analyses show that such antiviral activity is not mediated by interferons. Instead, adipocyte differentiation-induced antiviral activity is mediated by a factor that can be neutralized by polyclonal anti- C9 complement antibody. This factor is not C9 complement or ApoA1, the major proteins in adipocyte-conditioned media recognized by anti-C9 complement antibody, because those proteins in purified form do not possess assayable antiviral activity. Nonterminally differentiated adipocytes therefore produce a C9 complement-related antiviral factor and additional data show that this antiviral factor is stably expressed for many population doublings after adipocytes are induced to lose their differentiated phenotype and reinitiate proliferation.

Original languageEnglish (US)
Pages (from-to)199-210
Number of pages12
JournalMolecular and Cellular Differentiation
Volume4
Issue number2
StatePublished - Jan 1 1996

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Complement C9
Adipocytes
Antiviral Agents
Conditioned Culture Medium
Interferons
Phenotype
Antibodies
Mesenchymal Stromal Cells
Cell Cycle
Proteins

All Science Journal Classification (ASJC) codes

  • Genetics
  • Cell Biology
  • Cancer Research

Cite this

Adipocyte differentiation stably induces C9 complement- related antiviral activity. / Scott, R. E.; Tzen, C. Y.; Constantinescu, S. N.; Pfeffer, Lawrence.

In: Molecular and Cellular Differentiation, Vol. 4, No. 2, 01.01.1996, p. 199-210.

Research output: Contribution to journalArticle

Scott, R. E. ; Tzen, C. Y. ; Constantinescu, S. N. ; Pfeffer, Lawrence. / Adipocyte differentiation stably induces C9 complement- related antiviral activity. In: Molecular and Cellular Differentiation. 1996 ; Vol. 4, No. 2. pp. 199-210.
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