Adjuvanticity of an IL-12 fusion protein expressed by recombinant ΔG-vesicular stomatitis virus

Sheri D. Klas, Clinton S. Robison, Michael Whitt, Mark Miller

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The remarkable immunomodulatory and adjuvant properties of rIL-12 have been well described. Many early studies documenting the adjuvanticity of IL-12 were performed using the murine model of Listeria monocytogenes infection. In this report, we describe the construction of an attenuated recombinant vesicular stomatitis virus (VSV-ΔG) that encodes a single-chain IL-12 fusion protein (IL-12F), and the use of this virus as an expression vector to produce large quantities of IL-12F. VSV-expressed IL-12F (vIL-12F) was then co-administered to mice along with a poorly immunogenic listerial antigen preparation as a vaccine regimen and the resulting immune responses were monitored. The vIL-12F was found to have adjuvant properties similar to those observed for rIL-12. Co-administration of vIL-12F and listerial antigen elicited powerful cell-mediated immune responses that conferred long-lived protective listerial immunity. These studies demonstrated that VSVΔG-IL12F-infected cells secrete bioactive single-chain IL-12, and laid the foundation for studies using VSVΔG-IL12F as a vector for delivery of IL-12F in vivo.

Original languageEnglish (US)
Pages (from-to)59-73
Number of pages15
JournalCellular Immunology
Volume218
Issue number1-2
DOIs
StatePublished - Dec 1 2002

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Recombinant Fusion Proteins
Vesicular Stomatitis
Interleukin-12
Viruses
Viral Fusion Proteins
Antigens
Listeriosis
Proteins
Listeria monocytogenes
Immunity
Vaccines

All Science Journal Classification (ASJC) codes

  • Immunology

Cite this

Adjuvanticity of an IL-12 fusion protein expressed by recombinant ΔG-vesicular stomatitis virus. / Klas, Sheri D.; Robison, Clinton S.; Whitt, Michael; Miller, Mark.

In: Cellular Immunology, Vol. 218, No. 1-2, 01.12.2002, p. 59-73.

Research output: Contribution to journalArticle

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