Adult epicardial fat exhibits beige features

Harold S. Sacks, John N. Fain, Suleiman Bahouth, Shalini Ojha, Andrea Frontini, Helen Budge, Saverio Cinti, Michael E. Symonds

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

Context: Human epicardial fat has been designated previously as brown-like fat. The supraclavicular fat depot in man has been defined as beige coexistent with classical brown based on its gene expression profile. Objective: The aim of the study was to establish the gene expression profile and morphology of human epicardial and visceral paracardial fat compared with sc fat. Setting: The study was conducted at a tertiary care hospital cardiac center. Patients: Epicardial, visceral paracardial, and sc fat samples had been taken from middle-aged patients with severe coronary atherosclerosis or valvular heart disease. Interventions: Gene expression was determined by reverse transcription- quantitative PCR and relative abundance of the mitochondrial uncoupling protein-1 (UCP-1) by Western blotting. Epicardial tissue sections from patients were examined by light microscopy, UCP-1 immunohistochemistry, and cell morphometry. Main Outcome Measures:Wehypothesized that epicardial fat has a mixed phenotype with a gene expression profile similar to that described for beige cell lineage. Results: Immunoreactive UCP-1 was clearly measurable in each epicardial sample analyzed but was undetectable in each of the 4 other visceral and sc depots. Epicardial fat exhibited high expression of genes for UCP-1, PRDM16, PGC-1, PPAR, and the beige adipocyte-specific marker CD137, which were also expressed in visceral paracardial fat but only weakly in sternal, upper abdominal, and lower extremity sc fat. Histology of epicardial fat showed small unilocular adipocytes without UCP-1 immunostaining. Conclusion: UCP-1 is relatively abundant in epicardial fat, and this depot possesses molecular features characteristic of those found in vitro in beige lineage adipocytes.

Original languageEnglish (US)
JournalJournal of Clinical Endocrinology and Metabolism
Volume98
Issue number9
DOIs
StatePublished - Sep 1 2013

Fingerprint

Fats
Transcriptome
Gene expression
Intra-Abdominal Fat
Proteins
Beige Adipose Tissue
Heart Valve Diseases
Peroxisome Proliferator-Activated Receptors
Brown Adipose Tissue
Cell Lineage
Tertiary Healthcare
Adipocytes
Tertiary Care Centers
Reverse Transcription
Histology
Coronary Disease
Uncoupling Protein 1
Coronary Artery Disease
Microscopy
Lower Extremity

All Science Journal Classification (ASJC) codes

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Endocrinology
  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

Sacks, H. S., Fain, J. N., Bahouth, S., Ojha, S., Frontini, A., Budge, H., ... Symonds, M. E. (2013). Adult epicardial fat exhibits beige features. Journal of Clinical Endocrinology and Metabolism, 98(9). https://doi.org/10.1210/jc.2013-1265

Adult epicardial fat exhibits beige features. / Sacks, Harold S.; Fain, John N.; Bahouth, Suleiman; Ojha, Shalini; Frontini, Andrea; Budge, Helen; Cinti, Saverio; Symonds, Michael E.

In: Journal of Clinical Endocrinology and Metabolism, Vol. 98, No. 9, 01.09.2013.

Research output: Contribution to journalArticle

Sacks, HS, Fain, JN, Bahouth, S, Ojha, S, Frontini, A, Budge, H, Cinti, S & Symonds, ME 2013, 'Adult epicardial fat exhibits beige features', Journal of Clinical Endocrinology and Metabolism, vol. 98, no. 9. https://doi.org/10.1210/jc.2013-1265
Sacks, Harold S. ; Fain, John N. ; Bahouth, Suleiman ; Ojha, Shalini ; Frontini, Andrea ; Budge, Helen ; Cinti, Saverio ; Symonds, Michael E. / Adult epicardial fat exhibits beige features. In: Journal of Clinical Endocrinology and Metabolism. 2013 ; Vol. 98, No. 9.
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