Advanced zinc-doped adhesives for high performance at the resin-carious dentin interface

Manuel Toledano, Raquel Osorio, Estrella Osorio, Franklin Garcia-Godoy, Manuel Toledano-Osorio, Fátima S. Aguilera

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The purpose of this study was to evaluate the remineralization ability of an etch-and-rinse Zn-doped resin applied on caries-affected dentin (CAD). CAD surfaces were subjected to: (i) 37% phosphoric acid (PA) or (ii) 0.5 M ethylenediaminetetraacetic acid (EDTA). 10 wt% ZnO nanoparticles or 2 wt% ZnCl2 were added into the adhesive Single Bond (SB), to create the following groups: PA+SB, PA+SB-ZnO, PA+SB-ZnCl2, EDTA+SB, EDTA+SB-ZnO, EDTA+SB-ZnCl2. Bonded interfaces were submitted to mechanical loading or stored during 24 h. Remineralization of the bonded interfaces was studied by AFM nano-indentation (hardness and Young׳s modulus), Raman spectroscopy [mapping with principal component analysis (PCA), and hierarchical cluster analysis (HCA)] and Masson׳s trichrome staining technique. Dentin samples treated with PA+SB-ZnO attained the highest values of nano-mechanical properties. Load cycling increased both mineralization and crystallographic maturity at the interface; this effect was specially noticed when using ZnCl2-doped resin in EDTA-treated carious dentin. Crosslinking attained higher frequencies indicating better conformation and organization of collagen in specimens treated with PA+SB-ZnO, after load cycling. Trichrome staining technique depicted a deeper demineralized dentin fringe that became reduced after loading, and it was not observable in EDTA+SB groups. Multivariate analysis confirmed de homogenizing effect of load cycling in the percentage of variances, traces of centroids and distribution of clusters, especially in specimens treated with EDTA+SB-ZnCl2.

Original languageEnglish (US)
Pages (from-to)247-267
Number of pages21
JournalJournal of the Mechanical Behavior of Biomedical Materials
Volume62
DOIs
StatePublished - Sep 1 2016

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Ethylenediaminetetraacetic acid
Phosphoric acid
Zinc
Adhesives
Resins
Edetic Acid
Cluster analysis
Nanoindentation
single bond
Collagen
Crosslinking
Principal component analysis
Raman spectroscopy
Conformations
Loads (forces)
Elastic moduli
Hardness
Nanoparticles
Mechanical properties
phosphoric acid

All Science Journal Classification (ASJC) codes

  • Biomaterials
  • Biomedical Engineering
  • Mechanics of Materials

Cite this

Advanced zinc-doped adhesives for high performance at the resin-carious dentin interface. / Toledano, Manuel; Osorio, Raquel; Osorio, Estrella; Garcia-Godoy, Franklin; Toledano-Osorio, Manuel; Aguilera, Fátima S.

In: Journal of the Mechanical Behavior of Biomedical Materials, Vol. 62, 01.09.2016, p. 247-267.

Research output: Contribution to journalArticle

Toledano, Manuel ; Osorio, Raquel ; Osorio, Estrella ; Garcia-Godoy, Franklin ; Toledano-Osorio, Manuel ; Aguilera, Fátima S. / Advanced zinc-doped adhesives for high performance at the resin-carious dentin interface. In: Journal of the Mechanical Behavior of Biomedical Materials. 2016 ; Vol. 62. pp. 247-267.
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