Age-related apoptotic responses to stretch-induced hypertrophy in quail slow-tonic skeletal muscle

Parco M. Siu, Stephen Alway

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

In the present study, we examined the responses of apoptosis and apoptotic regulatory factors to muscle hypertrophy induced by stretch overload in quail slow-tonic muscles. The wings from one side of young and aged Japanese quails were loaded by attaching a tube weight corresponding to 12% of the bird's body weight for 7 or 21 days. Muscle from the contralateral side served as the intraanimal control. Relative to the intraanimal contralateral control side, the muscle wet weight increased by 96% in young birds, whereas the muscle weight gain in aged birds was not significant after 7 days of loading. After 21 days of loading, muscle weight significantly increased by 179% and 102% in young and aged birds, respectively. Heat shock protein (HSP)72 and HSP27 protein contents in the loaded sides were higher than on the control sides exclusively in young birds after 7 days of loading. Compared with the contralateral control muscle, the extent of apoptotic DNA fragmentation and the total cytosolic apoptosis-inducing factor protein content were reduced in all loaded muscles except for the 7-day-loaded muscles from the aged birds. Bax protein content was diminished in the loaded muscle relative to the control side from all groups, whereas Bcl-2 protein content was reduced in the young and aged muscles after 21 days of loading. The total cytosolic cytochrome c protein content was decreased and the X chromosome-linked inhibitor of apoptosis protein content was elevated in 7- and 21-day-loaded muscles relative to the intraanimal control muscle from young birds. Furthermore, after 7 days of loading the muscles of aged birds, H2O2 content and the total cytosolic protein content of second mitochondrial activator of caspases/direct inhibitor of apoptosis-binding protein with low isoelectric point were elevated compared with the intraanimal control side. These data suggest that stretch overload-induced muscle hypertrophy is associated with changes in apoptosis in slow-tonic skeletal muscle. Moreover, discrepant apoptotic responses to muscle overload in young and aged muscles may account in part for the age-related decline in the capability for muscle hypertrophy.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Cell Physiology
Volume289
Issue number5 58-5
DOIs
StatePublished - Nov 1 2005
Externally publishedYes

Fingerprint

Quail
Hypertrophy
Skeletal Muscle
Muscles
Birds
Weights and Measures
X-Linked Inhibitor of Apoptosis Protein
HSP72 Heat-Shock Proteins
Apoptosis Inducing Factor
HSP27 Heat-Shock Proteins
Apoptosis
Inhibitor of Apoptosis Proteins
bcl-2-Associated X Protein
Coturnix
Apoptosis Regulatory Proteins
Proteins
Caspase Inhibitors
Isoelectric Point

All Science Journal Classification (ASJC) codes

  • Physiology
  • Cell Biology

Cite this

Age-related apoptotic responses to stretch-induced hypertrophy in quail slow-tonic skeletal muscle. / Siu, Parco M.; Alway, Stephen.

In: American Journal of Physiology - Cell Physiology, Vol. 289, No. 5 58-5, 01.11.2005.

Research output: Contribution to journalArticle

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