Aggregation of group A streptococci by human saliva and effect of saliva on streptococcal adherence to host cells

H. S. Courtney, D. L. Hasty

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

The aggregation of group A streptococci by whole, stimulated human saliva (WHS) and the effect of saliva on streptococcal adherence to host cells was investigated. WHS samples from 11 individuals were found to aggregate both M+ and M- group A streptococci to various degrees. The aggregating activity was sensitive to heat, EDTA, EGTA [ethylene glycol-bis(β-aminoethyl ether)-N,N,N',N'-tetraacetic acid], sodium dodecyl sulfate, and lipoteichoic acid. None of the simple sugars tested, mercaptoethanol, albumin, or nonionic detergents had any effect on aggregation. The aggregating activity of EDTA-treated saliva was restored by 0.1 mM Ca2+ and 1.0 mM Mn2+ but not by up to 5 mM Mg2+. Only streptococci from the stationary phase were aggregated. Hyaluronidase treatment of streptococci from the exponential phase of growth restored their ability to be aggregated, suggesting that the hyaluronic acid capsule interferes with agglutination. Adsorption of WHS by one strain of Streptococcus pyogenes removed aggregating activity for other strains of S. pyogenes and Streptococcus sanguis but not agglutinins for Escherichia coli, suggesting that the agglutinin is specific for certain gram-positive bacteria. Molecular sieve chromatography of WHS and identification of streptococcus-binding components of saliva suggest that either a glycoprotein of ~360 kDa or a mucin of saliva of >1,000 kDa mediates aggregation of streptococci. WHS also inhibited adherence of S. pyogenes to buccal epithelial cells.

Original languageEnglish (US)
Pages (from-to)1661-1666
Number of pages6
JournalInfection and Immunity
Volume59
Issue number5
StatePublished - Jan 1 1991

Fingerprint

Streptococcus
Saliva
Streptococcus pyogenes
Agglutinins
Edetic Acid
Streptococcus sanguis
Forensic Anthropology
Hyaluronoglucosaminidase
Ethylene Glycol
Mercaptoethanol
Cheek
Egtazic Acid
Agglutination
Gram-Positive Bacteria
Mucins
Hyaluronic Acid
Sodium Dodecyl Sulfate
Detergents
Ether
Adsorption

All Science Journal Classification (ASJC) codes

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

Cite this

Aggregation of group A streptococci by human saliva and effect of saliva on streptococcal adherence to host cells. / Courtney, H. S.; Hasty, D. L.

In: Infection and Immunity, Vol. 59, No. 5, 01.01.1991, p. 1661-1666.

Research output: Contribution to journalArticle

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