Aging affects response to cyclic tensile stretch: Paradigm for intervertebral disc degeneration

Hongsik Cho, Aman Seth, Jenna Warmbold, James T. Robertson, Karen Hasty

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Much evidence supports a fundamental role for mechanical forces in modulating differentiation, homeostasis, and remodelling of musculoskeletal cells. Little is known, however, regarding mechanobiology and gene expression of intervertebral disc (IVD) cells from older individuals. To characterise the effect of mechanical stimulation on cells from older discs, an in vitro study of IVD cells harvested from different aged pigs was conducted to measure extracellular matrix (ECM) gene expression in response to cyclic tensile stress (CTS). Gene expression of annulus fibrosus (AF) cells from IVDs of mature and older pigs was quantified for the predominant ECM genes; type I collagen, type II collagen and aggrecan, and matrix metalloproteinase 1 (MMP-1), a collagenase that degrades fibrillar collagens. AF cells cultured on flexible-bottom plates were stretched 10% at 0.5 Hz frequency. After 24 h, gene expression was assayed using reverse transcriptase polymerase chain reaction (RT-PCR). Basal mRNA levels without stretching for type II collagen and aggrecan were lower in older annular cells whereas MMP-1 levels were higher compared to mature cells. Following CTS, an adaptive response was elicited in annular cells from both age groups. ECM protein genes were upregulated, whereas MMP-1 was downregulated. The magnitude of response was significantly greater in older cells as compared to mature cells. These data suggest that the cells from the AF of older animals manifest lower basal levels of mRNA for type II collagen and aggrecan and higher levels of MMP-1 possibly due to decreased tensile stress experienced in vivo and is not the result of reduced capacity for response.

Original languageEnglish (US)
Pages (from-to)137-146
Number of pages10
JournalEuropean Cells and Materials
Volume22
DOIs
StatePublished - Jan 1 2011

Fingerprint

Intervertebral Disc Degeneration
Matrix Metalloproteinase 1
Aggrecans
Collagen
Gene expression
Collagen Type II
Aging of materials
Tensile stress
Genes
Fibrillar Collagens
Messenger RNA
Extracellular Matrix Proteins
Polymerase chain reaction
RNA-Directed DNA Polymerase
Collagenases
Gene Expression
Stretching
Intervertebral Disc
Animals
Cells

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biochemistry
  • Biomaterials
  • Biomedical Engineering
  • Cell Biology

Cite this

Aging affects response to cyclic tensile stretch : Paradigm for intervertebral disc degeneration. / Cho, Hongsik; Seth, Aman; Warmbold, Jenna; Robertson, James T.; Hasty, Karen.

In: European Cells and Materials, Vol. 22, 01.01.2011, p. 137-146.

Research output: Contribution to journalArticle

@article{8cbab56eafc649c185d9ce53558b97a3,
title = "Aging affects response to cyclic tensile stretch: Paradigm for intervertebral disc degeneration",
abstract = "Much evidence supports a fundamental role for mechanical forces in modulating differentiation, homeostasis, and remodelling of musculoskeletal cells. Little is known, however, regarding mechanobiology and gene expression of intervertebral disc (IVD) cells from older individuals. To characterise the effect of mechanical stimulation on cells from older discs, an in vitro study of IVD cells harvested from different aged pigs was conducted to measure extracellular matrix (ECM) gene expression in response to cyclic tensile stress (CTS). Gene expression of annulus fibrosus (AF) cells from IVDs of mature and older pigs was quantified for the predominant ECM genes; type I collagen, type II collagen and aggrecan, and matrix metalloproteinase 1 (MMP-1), a collagenase that degrades fibrillar collagens. AF cells cultured on flexible-bottom plates were stretched 10{\%} at 0.5 Hz frequency. After 24 h, gene expression was assayed using reverse transcriptase polymerase chain reaction (RT-PCR). Basal mRNA levels without stretching for type II collagen and aggrecan were lower in older annular cells whereas MMP-1 levels were higher compared to mature cells. Following CTS, an adaptive response was elicited in annular cells from both age groups. ECM protein genes were upregulated, whereas MMP-1 was downregulated. The magnitude of response was significantly greater in older cells as compared to mature cells. These data suggest that the cells from the AF of older animals manifest lower basal levels of mRNA for type II collagen and aggrecan and higher levels of MMP-1 possibly due to decreased tensile stress experienced in vivo and is not the result of reduced capacity for response.",
author = "Hongsik Cho and Aman Seth and Jenna Warmbold and Robertson, {James T.} and Karen Hasty",
year = "2011",
month = "1",
day = "1",
doi = "10.22203/eCM.v022a11",
language = "English (US)",
volume = "22",
pages = "137--146",
journal = "European Cells and Materials",
issn = "1473-2262",
publisher = "Swiss Society for Biomaterials",

}

TY - JOUR

T1 - Aging affects response to cyclic tensile stretch

T2 - Paradigm for intervertebral disc degeneration

AU - Cho, Hongsik

AU - Seth, Aman

AU - Warmbold, Jenna

AU - Robertson, James T.

AU - Hasty, Karen

PY - 2011/1/1

Y1 - 2011/1/1

N2 - Much evidence supports a fundamental role for mechanical forces in modulating differentiation, homeostasis, and remodelling of musculoskeletal cells. Little is known, however, regarding mechanobiology and gene expression of intervertebral disc (IVD) cells from older individuals. To characterise the effect of mechanical stimulation on cells from older discs, an in vitro study of IVD cells harvested from different aged pigs was conducted to measure extracellular matrix (ECM) gene expression in response to cyclic tensile stress (CTS). Gene expression of annulus fibrosus (AF) cells from IVDs of mature and older pigs was quantified for the predominant ECM genes; type I collagen, type II collagen and aggrecan, and matrix metalloproteinase 1 (MMP-1), a collagenase that degrades fibrillar collagens. AF cells cultured on flexible-bottom plates were stretched 10% at 0.5 Hz frequency. After 24 h, gene expression was assayed using reverse transcriptase polymerase chain reaction (RT-PCR). Basal mRNA levels without stretching for type II collagen and aggrecan were lower in older annular cells whereas MMP-1 levels were higher compared to mature cells. Following CTS, an adaptive response was elicited in annular cells from both age groups. ECM protein genes were upregulated, whereas MMP-1 was downregulated. The magnitude of response was significantly greater in older cells as compared to mature cells. These data suggest that the cells from the AF of older animals manifest lower basal levels of mRNA for type II collagen and aggrecan and higher levels of MMP-1 possibly due to decreased tensile stress experienced in vivo and is not the result of reduced capacity for response.

AB - Much evidence supports a fundamental role for mechanical forces in modulating differentiation, homeostasis, and remodelling of musculoskeletal cells. Little is known, however, regarding mechanobiology and gene expression of intervertebral disc (IVD) cells from older individuals. To characterise the effect of mechanical stimulation on cells from older discs, an in vitro study of IVD cells harvested from different aged pigs was conducted to measure extracellular matrix (ECM) gene expression in response to cyclic tensile stress (CTS). Gene expression of annulus fibrosus (AF) cells from IVDs of mature and older pigs was quantified for the predominant ECM genes; type I collagen, type II collagen and aggrecan, and matrix metalloproteinase 1 (MMP-1), a collagenase that degrades fibrillar collagens. AF cells cultured on flexible-bottom plates were stretched 10% at 0.5 Hz frequency. After 24 h, gene expression was assayed using reverse transcriptase polymerase chain reaction (RT-PCR). Basal mRNA levels without stretching for type II collagen and aggrecan were lower in older annular cells whereas MMP-1 levels were higher compared to mature cells. Following CTS, an adaptive response was elicited in annular cells from both age groups. ECM protein genes were upregulated, whereas MMP-1 was downregulated. The magnitude of response was significantly greater in older cells as compared to mature cells. These data suggest that the cells from the AF of older animals manifest lower basal levels of mRNA for type II collagen and aggrecan and higher levels of MMP-1 possibly due to decreased tensile stress experienced in vivo and is not the result of reduced capacity for response.

UR - http://www.scopus.com/inward/record.url?scp=84857127632&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84857127632&partnerID=8YFLogxK

U2 - 10.22203/eCM.v022a11

DO - 10.22203/eCM.v022a11

M3 - Article

C2 - 21932191

AN - SCOPUS:84857127632

VL - 22

SP - 137

EP - 146

JO - European Cells and Materials

JF - European Cells and Materials

SN - 1473-2262

ER -