Aging alters the reduction of pro-apoptotic signaling in response to loading-induced hypertrophy

Parco M. Siu, Stephen Alway

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

This study tested the hypothesis that loading decreased apoptosis in skeletal muscle in an aging-dependent fashion. One wing of young and aged Japanese quails was loaded for 7 or 21 days to induce hypertrophy. The contralateral wing served as the intra-animal control. Loading increased fast-twitch quail patagialis muscle mass by 28 and 49%, after 7 or 21 days of loading, respectively in young adult birds. Muscle mass was not elevated after 7 days of loading, but increased by 29% after 21 days of loading in aged birds. Seven days of loading reduced DNA fragmentation and cytosolic accumulation of cytochrome c in muscles from young birds but not in muscles from aged birds. ARC protein content was lower and H2O2 content was higher in muscles from aged birds following 7 days of loading. The mitochondria-free cytosolic protein fraction from muscles loaded for 7 days had 41 and 29% lower AIF content than control muscles in young and aged birds, respectively. XIAP, an apoptotic suppressor protein increased after 7 days of loading in muscles from young adult but not aged birds. Our results suggest that loading suppresses pro-apoptotic signaling in quail muscle but aging delays or attenuates these anti-apoptotic changes.

Original languageEnglish (US)
Pages (from-to)175-188
Number of pages14
JournalExperimental Gerontology
Volume41
Issue number2
DOIs
StatePublished - Feb 1 2006
Externally publishedYes

Fingerprint

Hypertrophy
Muscle
Birds
Aging of materials
Muscles
Quail
Young Adult
AIDS-Related Complex
Coturnix
Muscle Proteins
DNA Fragmentation
Cytochromes c
Proteins
Mitochondria
Skeletal Muscle
Apoptosis
Animals
DNA

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Aging
  • Molecular Biology
  • Genetics
  • Endocrinology
  • Cell Biology

Cite this

Aging alters the reduction of pro-apoptotic signaling in response to loading-induced hypertrophy. / Siu, Parco M.; Alway, Stephen.

In: Experimental Gerontology, Vol. 41, No. 2, 01.02.2006, p. 175-188.

Research output: Contribution to journalArticle

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