Aging-associated attenuation of p53, Rb and PDGF receptor proteins in stretched quail skeletal muscle

Stephen Alway, T. R. Samelman, P. A. Lowe

Research output: Contribution to journalArticle

Abstract

Stretch-induced hypertrophy and new fiber formation are lower in anterior latissimus dorsi (ALD) muscles from aged quail when compared to young adult quail. Stretch-induced muscle growth is dependent upon activation and proliferation of satellite cells; however, it is not known if nuclear regulatory proteins are lower in vivo in aged muscles during stretch-overload. To test this hypothesis we examined whether aging would attenuate stretch-induced ALD protein levels of platelet derived growth factor (PDGF) and PDGF receptors (PDGF-R); cell cycle regulators p53 and retinoblastoma protein (Rb); and the 70 kD heat shock chaperon protein family (HSP72/73), relative to young quail. The left wing of adult (8 weeks of age) and aged (24 months of age) birds were weighted for 0, 7, 14, or 21 days with 10% of their body weight, and the right wing served as the control. Immunoblot analyses of ALD muscles showed that relative protein levels of PDGF-A and PDGF-B did not increased with stretch in either age group; however, stretch increased the relative protein levels of PDGF-R(α) and PDGF-R(β), p53, Rb, and HSP72/73. Aging attenuated these increases. These data are consistent with the hypothesis that the aging-reduced proliferation and commitment of satellite cells to form new fibers during stretch is associated with reduced levels of nuclear regulator proteins in aged quail muscles. Funded by NIH AG10871.

Original languageEnglish (US)
JournalFASEB Journal
Volume11
Issue number3
StatePublished - Dec 1 1997
Externally publishedYes

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Platelet-Derived Growth Factor Receptors
Quail
Muscle
Skeletal Muscle
Aging of materials
Superficial Back Muscles
Platelet-Derived Growth Factor
Muscles
Nuclear Proteins
Proteins
Proto-Oncogene Proteins c-sis
Retinoblastoma Protein
Satellites
Heat-Shock Proteins
Fibers
Hypertrophy
Birds
Young Adult
Cell Cycle
Age Groups

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

Cite this

Aging-associated attenuation of p53, Rb and PDGF receptor proteins in stretched quail skeletal muscle. / Alway, Stephen; Samelman, T. R.; Lowe, P. A.

In: FASEB Journal, Vol. 11, No. 3, 01.12.1997.

Research output: Contribution to journalArticle

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