Airway Epithelial Repair by a Prebiotic Mannan Derived from Saccharomyces cerevisiae

Christie Michael, Christopher M. Waters, Kim S. Lemessurier, Amali Samarasinghe, Chi Y. Song, Kafait Malik, D. Betty Lew

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

In asthmatic airways, repeated epithelial damage and repair occur. No current therapy directly targets this process. We aimed to determine the effects of mannan derived from S. cerevisiae (SC-MN) on airway epithelial wound repair, in vitro. The presence of functional mannose receptors in bronchial epithelial cells was shown by endocytosis of colloidal gold-Man BSA via clathrin-coated pits in 16HBE cells. In primary normal human bronchial epithelial cells (NHBEC), SC-MN significantly facilitated wound closure. Treatment with SC-MN stimulated cell spreading as indicated by a significant increase in the average lamellipodial width of wound edge 16HBE cells. In addition, NHBEC treated with SC-MN showed increased expression and activation of Krüppel-like factors (KLFs) 4 and 5, transcription factors important in epithelial cell survival and regulation of epithelial-mesenchymal transition. We conclude that SC-MN facilitates wound repair in human bronchial epithelium, involving mannose receptors.

Original languageEnglish (US)
Article number8903982
JournalJournal of Immunology Research
Volume2017
DOIs
StatePublished - Jan 1 2017

Fingerprint

Mannans
Prebiotics
Saccharomyces cerevisiae
Epithelial Cells
Wounds and Injuries
Gold Colloid
Clathrin
Epithelial-Mesenchymal Transition
Endocytosis
Cell Survival
Transcription Factors
Epithelium
mannose receptor
Therapeutics

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

Airway Epithelial Repair by a Prebiotic Mannan Derived from Saccharomyces cerevisiae. / Michael, Christie; Waters, Christopher M.; Lemessurier, Kim S.; Samarasinghe, Amali; Song, Chi Y.; Malik, Kafait; Lew, D. Betty.

In: Journal of Immunology Research, Vol. 2017, 8903982, 01.01.2017.

Research output: Contribution to journalArticle

@article{8b73fe531c7841ef849f081bd8e9d32a,
title = "Airway Epithelial Repair by a Prebiotic Mannan Derived from Saccharomyces cerevisiae",
abstract = "In asthmatic airways, repeated epithelial damage and repair occur. No current therapy directly targets this process. We aimed to determine the effects of mannan derived from S. cerevisiae (SC-MN) on airway epithelial wound repair, in vitro. The presence of functional mannose receptors in bronchial epithelial cells was shown by endocytosis of colloidal gold-Man BSA via clathrin-coated pits in 16HBE cells. In primary normal human bronchial epithelial cells (NHBEC), SC-MN significantly facilitated wound closure. Treatment with SC-MN stimulated cell spreading as indicated by a significant increase in the average lamellipodial width of wound edge 16HBE cells. In addition, NHBEC treated with SC-MN showed increased expression and activation of Kr{\"u}ppel-like factors (KLFs) 4 and 5, transcription factors important in epithelial cell survival and regulation of epithelial-mesenchymal transition. We conclude that SC-MN facilitates wound repair in human bronchial epithelium, involving mannose receptors.",
author = "Christie Michael and Waters, {Christopher M.} and Lemessurier, {Kim S.} and Amali Samarasinghe and Song, {Chi Y.} and Kafait Malik and Lew, {D. Betty}",
year = "2017",
month = "1",
day = "1",
doi = "10.1155/2017/8903982",
language = "English (US)",
volume = "2017",
journal = "Journal of Immunology Research",
issn = "2314-8861",
publisher = "Hindawi Publishing Corporation",

}

TY - JOUR

T1 - Airway Epithelial Repair by a Prebiotic Mannan Derived from Saccharomyces cerevisiae

AU - Michael, Christie

AU - Waters, Christopher M.

AU - Lemessurier, Kim S.

AU - Samarasinghe, Amali

AU - Song, Chi Y.

AU - Malik, Kafait

AU - Lew, D. Betty

PY - 2017/1/1

Y1 - 2017/1/1

N2 - In asthmatic airways, repeated epithelial damage and repair occur. No current therapy directly targets this process. We aimed to determine the effects of mannan derived from S. cerevisiae (SC-MN) on airway epithelial wound repair, in vitro. The presence of functional mannose receptors in bronchial epithelial cells was shown by endocytosis of colloidal gold-Man BSA via clathrin-coated pits in 16HBE cells. In primary normal human bronchial epithelial cells (NHBEC), SC-MN significantly facilitated wound closure. Treatment with SC-MN stimulated cell spreading as indicated by a significant increase in the average lamellipodial width of wound edge 16HBE cells. In addition, NHBEC treated with SC-MN showed increased expression and activation of Krüppel-like factors (KLFs) 4 and 5, transcription factors important in epithelial cell survival and regulation of epithelial-mesenchymal transition. We conclude that SC-MN facilitates wound repair in human bronchial epithelium, involving mannose receptors.

AB - In asthmatic airways, repeated epithelial damage and repair occur. No current therapy directly targets this process. We aimed to determine the effects of mannan derived from S. cerevisiae (SC-MN) on airway epithelial wound repair, in vitro. The presence of functional mannose receptors in bronchial epithelial cells was shown by endocytosis of colloidal gold-Man BSA via clathrin-coated pits in 16HBE cells. In primary normal human bronchial epithelial cells (NHBEC), SC-MN significantly facilitated wound closure. Treatment with SC-MN stimulated cell spreading as indicated by a significant increase in the average lamellipodial width of wound edge 16HBE cells. In addition, NHBEC treated with SC-MN showed increased expression and activation of Krüppel-like factors (KLFs) 4 and 5, transcription factors important in epithelial cell survival and regulation of epithelial-mesenchymal transition. We conclude that SC-MN facilitates wound repair in human bronchial epithelium, involving mannose receptors.

UR - http://www.scopus.com/inward/record.url?scp=85026521929&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85026521929&partnerID=8YFLogxK

U2 - 10.1155/2017/8903982

DO - 10.1155/2017/8903982

M3 - Article

VL - 2017

JO - Journal of Immunology Research

JF - Journal of Immunology Research

SN - 2314-8861

M1 - 8903982

ER -