Allantoin transport in Saccharomyces cerevisiae

R. Sumrada, Terrance Cooper

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Allantoin uptake in both growing and resting cultures of S. cerevisiae occurs by a low K(m) (ca. 15 μM) transport system that uses energy that is likely generated in the cytoplasm. This conclusion was based on the observation that transport did not occur in the absence of glucose or the presence of dinitrophenol, carbonyl cyanide-m-chloro-phenyl hydrazone, fluoride, or arsenate ions. Normal uptake was observed, however, in the presence of cyanide. The rate of accumulation was maximal at pH 5.2. In contrast to the urea transport system, allantoin uptake appeared to be unidirectional. Preloaded, radioactive allantoin was not lost from cells suspended in allantoin-free buffer and did not exchange with exogenously added, nonradioactive allantoin. Treatment of preloaded cells with nystatin, however, released the accumulated radioactivity. Allantoin accumulated within cells was isolated and shown to be chemically unaltered.

Original languageEnglish (US)
Pages (from-to)839-847
Number of pages9
JournalJournal of Bacteriology
Volume131
Issue number3
StatePublished - Dec 1 1977
Externally publishedYes

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Allantoin
Saccharomyces cerevisiae
Dinitrophenols
Nystatin
Hydrazones
Cyanides
Fluorides
Radioactivity
Urea
Buffers
Cytoplasm
Ions
Glucose

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology

Cite this

Allantoin transport in Saccharomyces cerevisiae. / Sumrada, R.; Cooper, Terrance.

In: Journal of Bacteriology, Vol. 131, No. 3, 01.12.1977, p. 839-847.

Research output: Contribution to journalArticle

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