Allelotyping of Hras1 Minisatellite

Formation of Carcinogenic Risk Groups and Prognosis of the Clinical Course of Non-Small Cell Lung Cancer

Viktor Chizhikov, A. V. Gasparian, I. V. Zborovskaya

Research output: Contribution to journalArticle

Abstract

PCR-based typing of Hras1 minisatellite alleles was carried out in 226 non-small cell lung cancer (NSCLC) patients and 207 unaffected controls. Application of this method permitted detection of four common (a1 to a4) and 25 other alleles, differing from any common allele by one or more repeat units. Depending on their frequency in control group, these alleles were defined as intermediate or rare (the frequency over 0.5% or less than 0.5%, respectively). It was established that the frequency of rare alleles in the group of NSCLC patients (7.1%) was statistically significantly higher than in healthy individuals (2.2%, P = 0.002), while the difference in the distribution of common and intermediate alleles between the compared groups was not statistically significant. In addition, rare Hras1 alleles were more frequent (P = 0.02) among nonsmoking patients (P = 0.02) compared to the patients subjected to of tobacco carcinogens. The presence of "heavy" (a3-a4) alleles was associated with an increased risk of low-differentiated and/or actively metastasizing tumors and also with the risk of lung cancer in the patients under 50 years of age (P < 0.05). These data indicate that an approach including application of modern highly sensitive techniques of Hras1 allele typing in combination with preliminary examination of healthy control population can be employed for identifying carcinogenic risk groups as well as for prognosis of the NSCLC clinical course.

Original languageEnglish (US)
Pages (from-to)1165-1171
Number of pages7
JournalRussian Journal of Genetics
Volume37
Issue number10
DOIs
StatePublished - Oct 1 2001

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Minisatellite Repeats
Non-Small Cell Lung Carcinoma
Alleles
Gene Frequency
Carcinogens
Tobacco
Lung Neoplasms
Polymerase Chain Reaction
Control Groups

All Science Journal Classification (ASJC) codes

  • Genetics

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Allelotyping of Hras1 Minisatellite : Formation of Carcinogenic Risk Groups and Prognosis of the Clinical Course of Non-Small Cell Lung Cancer. / Chizhikov, Viktor; Gasparian, A. V.; Zborovskaya, I. V.

In: Russian Journal of Genetics, Vol. 37, No. 10, 01.10.2001, p. 1165-1171.

Research output: Contribution to journalArticle

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