Allicin-Induced Hypotension in Rabbit Eyes

Teh Ching Chu, Miller Ogidigben, Joan Han, David E. Potter

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The intent of this work was to examine the actions of allicin on 1) intraocular pressure(IOP) in normal and unilaterally sympathectomized (SX) rabbits; 2) cAMP accumulation in the rabbit iris-ciliary body(ICB) and cultured nonpigmented epithelial(NPE) ciliary body cells; and 3) 3H-norepinephrine (NE) release by calculating fractional tritium overflow in response to electrical field stimulation(EFS, 5 Hz, 12 V/cm) in isolated, perfused rabbit ICBs. Allicin, one of the active compounds produced by garlic, was evaluated on IOP and it was determined that allicin (1, 2.5, or 10 ug), topically, but not the precursor, alliin (10 ug), lowered the IOP unilaterally in normal rabbits. Allicin(10 ug) reduced the IOP by 6±1 mmHg (n=4) in normal rabbits at 2 hrs(maximum response) whereas no change occurred in sympathectomized rabbit eyes. Moreover, allicin (0.01, 0.1, or 1 uM) caused 40, 40, or 52% inhibition, respectively, of 3H-NE overflow in response to EFS. Isoproterenol (ISO, 1 uM) stimulated cAMP accumulation by 3.6 and 9 fold in isolated rabbit ICB and cultured NPE cells, respectively. Allicin(1 uM) had no effect on basal cAMP level while it inhibited ISO-stimulated cAMP accumulation by 40% and 23% in ICB and NPE cells, respectively. This study suggests that allicin lowered IOP, in part, by dual actions at the neuroeffector junction.

Original languageEnglish (US)
Pages (from-to)201-209
Number of pages9
JournalJournal of Ocular Pharmacology
Volume9
Issue number3
DOIs
StatePublished - Jan 1 1993
Externally publishedYes

Fingerprint

Controlled Hypotension
Intraocular Pressure
Ciliary Body
Rabbits
Iris
Norepinephrine
Neuroeffector Junction
Epithelial Cells
Garlic
Tritium
allicin
Isoproterenol
Electric Stimulation

All Science Journal Classification (ASJC) codes

  • Ophthalmology
  • Pharmacology
  • Pharmacology (medical)

Cite this

Allicin-Induced Hypotension in Rabbit Eyes. / Chu, Teh Ching; Ogidigben, Miller; Han, Joan; Potter, David E.

In: Journal of Ocular Pharmacology, Vol. 9, No. 3, 01.01.1993, p. 201-209.

Research output: Contribution to journalArticle

Chu, Teh Ching ; Ogidigben, Miller ; Han, Joan ; Potter, David E. / Allicin-Induced Hypotension in Rabbit Eyes. In: Journal of Ocular Pharmacology. 1993 ; Vol. 9, No. 3. pp. 201-209.
@article{66bd70cb060d49d2aefa93e3e8283fba,
title = "Allicin-Induced Hypotension in Rabbit Eyes",
abstract = "The intent of this work was to examine the actions of allicin on 1) intraocular pressure(IOP) in normal and unilaterally sympathectomized (SX) rabbits; 2) cAMP accumulation in the rabbit iris-ciliary body(ICB) and cultured nonpigmented epithelial(NPE) ciliary body cells; and 3) 3H-norepinephrine (NE) release by calculating fractional tritium overflow in response to electrical field stimulation(EFS, 5 Hz, 12 V/cm) in isolated, perfused rabbit ICBs. Allicin, one of the active compounds produced by garlic, was evaluated on IOP and it was determined that allicin (1, 2.5, or 10 ug), topically, but not the precursor, alliin (10 ug), lowered the IOP unilaterally in normal rabbits. Allicin(10 ug) reduced the IOP by 6±1 mmHg (n=4) in normal rabbits at 2 hrs(maximum response) whereas no change occurred in sympathectomized rabbit eyes. Moreover, allicin (0.01, 0.1, or 1 uM) caused 40, 40, or 52{\%} inhibition, respectively, of 3H-NE overflow in response to EFS. Isoproterenol (ISO, 1 uM) stimulated cAMP accumulation by 3.6 and 9 fold in isolated rabbit ICB and cultured NPE cells, respectively. Allicin(1 uM) had no effect on basal cAMP level while it inhibited ISO-stimulated cAMP accumulation by 40{\%} and 23{\%} in ICB and NPE cells, respectively. This study suggests that allicin lowered IOP, in part, by dual actions at the neuroeffector junction.",
author = "Chu, {Teh Ching} and Miller Ogidigben and Joan Han and Potter, {David E.}",
year = "1993",
month = "1",
day = "1",
doi = "10.1089/jop.1993.9.201",
language = "English (US)",
volume = "9",
pages = "201--209",
journal = "Journal of Ocular Pharmacology and Therapeutics",
issn = "1080-7683",
publisher = "Mary Ann Liebert Inc.",
number = "3",

}

TY - JOUR

T1 - Allicin-Induced Hypotension in Rabbit Eyes

AU - Chu, Teh Ching

AU - Ogidigben, Miller

AU - Han, Joan

AU - Potter, David E.

PY - 1993/1/1

Y1 - 1993/1/1

N2 - The intent of this work was to examine the actions of allicin on 1) intraocular pressure(IOP) in normal and unilaterally sympathectomized (SX) rabbits; 2) cAMP accumulation in the rabbit iris-ciliary body(ICB) and cultured nonpigmented epithelial(NPE) ciliary body cells; and 3) 3H-norepinephrine (NE) release by calculating fractional tritium overflow in response to electrical field stimulation(EFS, 5 Hz, 12 V/cm) in isolated, perfused rabbit ICBs. Allicin, one of the active compounds produced by garlic, was evaluated on IOP and it was determined that allicin (1, 2.5, or 10 ug), topically, but not the precursor, alliin (10 ug), lowered the IOP unilaterally in normal rabbits. Allicin(10 ug) reduced the IOP by 6±1 mmHg (n=4) in normal rabbits at 2 hrs(maximum response) whereas no change occurred in sympathectomized rabbit eyes. Moreover, allicin (0.01, 0.1, or 1 uM) caused 40, 40, or 52% inhibition, respectively, of 3H-NE overflow in response to EFS. Isoproterenol (ISO, 1 uM) stimulated cAMP accumulation by 3.6 and 9 fold in isolated rabbit ICB and cultured NPE cells, respectively. Allicin(1 uM) had no effect on basal cAMP level while it inhibited ISO-stimulated cAMP accumulation by 40% and 23% in ICB and NPE cells, respectively. This study suggests that allicin lowered IOP, in part, by dual actions at the neuroeffector junction.

AB - The intent of this work was to examine the actions of allicin on 1) intraocular pressure(IOP) in normal and unilaterally sympathectomized (SX) rabbits; 2) cAMP accumulation in the rabbit iris-ciliary body(ICB) and cultured nonpigmented epithelial(NPE) ciliary body cells; and 3) 3H-norepinephrine (NE) release by calculating fractional tritium overflow in response to electrical field stimulation(EFS, 5 Hz, 12 V/cm) in isolated, perfused rabbit ICBs. Allicin, one of the active compounds produced by garlic, was evaluated on IOP and it was determined that allicin (1, 2.5, or 10 ug), topically, but not the precursor, alliin (10 ug), lowered the IOP unilaterally in normal rabbits. Allicin(10 ug) reduced the IOP by 6±1 mmHg (n=4) in normal rabbits at 2 hrs(maximum response) whereas no change occurred in sympathectomized rabbit eyes. Moreover, allicin (0.01, 0.1, or 1 uM) caused 40, 40, or 52% inhibition, respectively, of 3H-NE overflow in response to EFS. Isoproterenol (ISO, 1 uM) stimulated cAMP accumulation by 3.6 and 9 fold in isolated rabbit ICB and cultured NPE cells, respectively. Allicin(1 uM) had no effect on basal cAMP level while it inhibited ISO-stimulated cAMP accumulation by 40% and 23% in ICB and NPE cells, respectively. This study suggests that allicin lowered IOP, in part, by dual actions at the neuroeffector junction.

UR - http://www.scopus.com/inward/record.url?scp=0027486478&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027486478&partnerID=8YFLogxK

U2 - 10.1089/jop.1993.9.201

DO - 10.1089/jop.1993.9.201

M3 - Article

VL - 9

SP - 201

EP - 209

JO - Journal of Ocular Pharmacology and Therapeutics

JF - Journal of Ocular Pharmacology and Therapeutics

SN - 1080-7683

IS - 3

ER -