Alterations in hippocampal serotonergic and INSR function in streptozotocin induced diabetic rats exposed to stress: Neuroprotective role of pyridoxine and Aegle marmelose

Pretty Mary Abraham, Korah Pushpamangalam Kuruvilla, Jobin Mathew, Anitha Malat, Shilpa Joy, C. S. Paulose

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Diabetes and stress stimulate hippocampal 5-HT synthesis, metabolism and release. The present study was carried out to find the effects of insulin, Aegle marmelose alone and in combination with pyridoxine on the hippocampal 5-HT, 5-HT2A receptor subtype, gene expression studies on 5-HT 2A, 5-HTT, INSR, immunohistochemical studies and elevated plus maze in streptozotocin induced diabetic rats. 5-HT content showed a significant decrease (p < 0.001) and a significant increase (p < 0.001) in 5-HIAA in hippocampus of diabetic rats compared to control. 5-HT receptor binding parameters Bmax and Kd showed a significant decrease (p < 0.001) whereas 5-HT2A receptor binding parameters B max showed a significant decrease (p < 0.001) with a significant increase (p < 0.05) in Kd in hippocampus of diabetic rats compared to control. Gene expression studies of 5-HT2A 5-HTT and INSR in hippocampus showed a significant down regulation (p < 0.001) in diabetic rats compared to control. Pyridoxine treated in combination with insulin and A. marmelose to diabetic rats reversed the 5-HT content, Bmax, K dof 5-HT, 5-HT2Aand gene expression of 5-HT2A, 5-HTT and INSR in hippocampus to near control. The gene expression of 5-HT 2A and 5-HTT were confirmed by immunohistochemical studies. Behavioural studies using elevated plus maze showed that serotonin through its transporter significantly increased (p < 0.001) anxiety-related traits in diabetic rats which were corrected by combination therapy. Our results suggest that pyridoxine treated in combination with insulin and A. marmelose has a role in the regulation of insulin synthesis and release, normalising diabetic related stress and anxiety through hippocampal serotonergic function. This has clinical significance in the management of diabetes.

Original languageEnglish (US)
Article number78
JournalJournal of Biomedical Science
Volume17
Issue number1
DOIs
StatePublished - Sep 28 2010

Fingerprint

Aegle
Pyridoxine
Streptozocin
Rats
Serotonin
Gene expression
Hippocampus
Insulin
Gene Expression
Receptor, Serotonin, 5-HT2A
Medical problems
Anxiety
Hydroxyindoleacetic Acid
Serotonin Receptors
Metabolism
Down-Regulation

All Science Journal Classification (ASJC) codes

  • Endocrinology, Diabetes and Metabolism
  • Molecular Biology
  • Clinical Biochemistry
  • Cell Biology
  • Biochemistry, medical
  • Pharmacology (medical)

Cite this

Alterations in hippocampal serotonergic and INSR function in streptozotocin induced diabetic rats exposed to stress : Neuroprotective role of pyridoxine and Aegle marmelose. / Abraham, Pretty Mary; Pushpamangalam Kuruvilla, Korah; Mathew, Jobin; Malat, Anitha; Joy, Shilpa; Paulose, C. S.

In: Journal of Biomedical Science, Vol. 17, No. 1, 78, 28.09.2010.

Research output: Contribution to journalArticle

@article{c8ab51880739448b939bba07ad42014a,
title = "Alterations in hippocampal serotonergic and INSR function in streptozotocin induced diabetic rats exposed to stress: Neuroprotective role of pyridoxine and Aegle marmelose",
abstract = "Diabetes and stress stimulate hippocampal 5-HT synthesis, metabolism and release. The present study was carried out to find the effects of insulin, Aegle marmelose alone and in combination with pyridoxine on the hippocampal 5-HT, 5-HT2A receptor subtype, gene expression studies on 5-HT 2A, 5-HTT, INSR, immunohistochemical studies and elevated plus maze in streptozotocin induced diabetic rats. 5-HT content showed a significant decrease (p < 0.001) and a significant increase (p < 0.001) in 5-HIAA in hippocampus of diabetic rats compared to control. 5-HT receptor binding parameters Bmax and Kd showed a significant decrease (p < 0.001) whereas 5-HT2A receptor binding parameters B max showed a significant decrease (p < 0.001) with a significant increase (p < 0.05) in Kd in hippocampus of diabetic rats compared to control. Gene expression studies of 5-HT2A 5-HTT and INSR in hippocampus showed a significant down regulation (p < 0.001) in diabetic rats compared to control. Pyridoxine treated in combination with insulin and A. marmelose to diabetic rats reversed the 5-HT content, Bmax, K dof 5-HT, 5-HT2Aand gene expression of 5-HT2A, 5-HTT and INSR in hippocampus to near control. The gene expression of 5-HT 2A and 5-HTT were confirmed by immunohistochemical studies. Behavioural studies using elevated plus maze showed that serotonin through its transporter significantly increased (p < 0.001) anxiety-related traits in diabetic rats which were corrected by combination therapy. Our results suggest that pyridoxine treated in combination with insulin and A. marmelose has a role in the regulation of insulin synthesis and release, normalising diabetic related stress and anxiety through hippocampal serotonergic function. This has clinical significance in the management of diabetes.",
author = "Abraham, {Pretty Mary} and {Pushpamangalam Kuruvilla}, Korah and Jobin Mathew and Anitha Malat and Shilpa Joy and Paulose, {C. S.}",
year = "2010",
month = "9",
day = "28",
doi = "10.1186/1423-0127-17-78",
language = "English (US)",
volume = "17",
journal = "Journal of Biomedical Science",
issn = "1021-7770",
publisher = "BioMed Central",
number = "1",

}

TY - JOUR

T1 - Alterations in hippocampal serotonergic and INSR function in streptozotocin induced diabetic rats exposed to stress

T2 - Neuroprotective role of pyridoxine and Aegle marmelose

AU - Abraham, Pretty Mary

AU - Pushpamangalam Kuruvilla, Korah

AU - Mathew, Jobin

AU - Malat, Anitha

AU - Joy, Shilpa

AU - Paulose, C. S.

PY - 2010/9/28

Y1 - 2010/9/28

N2 - Diabetes and stress stimulate hippocampal 5-HT synthesis, metabolism and release. The present study was carried out to find the effects of insulin, Aegle marmelose alone and in combination with pyridoxine on the hippocampal 5-HT, 5-HT2A receptor subtype, gene expression studies on 5-HT 2A, 5-HTT, INSR, immunohistochemical studies and elevated plus maze in streptozotocin induced diabetic rats. 5-HT content showed a significant decrease (p < 0.001) and a significant increase (p < 0.001) in 5-HIAA in hippocampus of diabetic rats compared to control. 5-HT receptor binding parameters Bmax and Kd showed a significant decrease (p < 0.001) whereas 5-HT2A receptor binding parameters B max showed a significant decrease (p < 0.001) with a significant increase (p < 0.05) in Kd in hippocampus of diabetic rats compared to control. Gene expression studies of 5-HT2A 5-HTT and INSR in hippocampus showed a significant down regulation (p < 0.001) in diabetic rats compared to control. Pyridoxine treated in combination with insulin and A. marmelose to diabetic rats reversed the 5-HT content, Bmax, K dof 5-HT, 5-HT2Aand gene expression of 5-HT2A, 5-HTT and INSR in hippocampus to near control. The gene expression of 5-HT 2A and 5-HTT were confirmed by immunohistochemical studies. Behavioural studies using elevated plus maze showed that serotonin through its transporter significantly increased (p < 0.001) anxiety-related traits in diabetic rats which were corrected by combination therapy. Our results suggest that pyridoxine treated in combination with insulin and A. marmelose has a role in the regulation of insulin synthesis and release, normalising diabetic related stress and anxiety through hippocampal serotonergic function. This has clinical significance in the management of diabetes.

AB - Diabetes and stress stimulate hippocampal 5-HT synthesis, metabolism and release. The present study was carried out to find the effects of insulin, Aegle marmelose alone and in combination with pyridoxine on the hippocampal 5-HT, 5-HT2A receptor subtype, gene expression studies on 5-HT 2A, 5-HTT, INSR, immunohistochemical studies and elevated plus maze in streptozotocin induced diabetic rats. 5-HT content showed a significant decrease (p < 0.001) and a significant increase (p < 0.001) in 5-HIAA in hippocampus of diabetic rats compared to control. 5-HT receptor binding parameters Bmax and Kd showed a significant decrease (p < 0.001) whereas 5-HT2A receptor binding parameters B max showed a significant decrease (p < 0.001) with a significant increase (p < 0.05) in Kd in hippocampus of diabetic rats compared to control. Gene expression studies of 5-HT2A 5-HTT and INSR in hippocampus showed a significant down regulation (p < 0.001) in diabetic rats compared to control. Pyridoxine treated in combination with insulin and A. marmelose to diabetic rats reversed the 5-HT content, Bmax, K dof 5-HT, 5-HT2Aand gene expression of 5-HT2A, 5-HTT and INSR in hippocampus to near control. The gene expression of 5-HT 2A and 5-HTT were confirmed by immunohistochemical studies. Behavioural studies using elevated plus maze showed that serotonin through its transporter significantly increased (p < 0.001) anxiety-related traits in diabetic rats which were corrected by combination therapy. Our results suggest that pyridoxine treated in combination with insulin and A. marmelose has a role in the regulation of insulin synthesis and release, normalising diabetic related stress and anxiety through hippocampal serotonergic function. This has clinical significance in the management of diabetes.

UR - http://www.scopus.com/inward/record.url?scp=77956905984&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77956905984&partnerID=8YFLogxK

U2 - 10.1186/1423-0127-17-78

DO - 10.1186/1423-0127-17-78

M3 - Article

C2 - 20868513

AN - SCOPUS:77956905984

VL - 17

JO - Journal of Biomedical Science

JF - Journal of Biomedical Science

SN - 1021-7770

IS - 1

M1 - 78

ER -