Alterations in hypertrophic gene expression by dietary copper restriction in mouse heart

Yujian Kang, Huiyun Wu, Jack T. Saari

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Dietary copper (Cu) restriction causes a hypertrophic cardiomyopathy similar to that induced by work overload in rodent models. However, a possible change in the program of hypertrophic gene expression has not been studied in the Cu-deficient heart. This study was undertaken to fill that gap. Dams of mouse pups were fed a Cu-deficient diet (0.35 mg/kg diet) or a Cu-adequate control diet (6.10 mg/kg) on the fourth day after birth, and weanling mice continued on the dams' diet until they were sacrificed. After 5 weeks of feeding, Cu concentrations were dramatically decreased in the heart and the liver of the mice fed the Cu-deficient diet. Corresponding to these changes, serum ceruloplasmin concentrations and hepatic Cu,Zn-superoxide dismutase activities were significantly (P < 0.05) depressed. The size of the Cu-deficient hearts was greatly enlarged as estimated from the absolute heart weight and the ratio of heart weight to body weight. The abundances of mRNAs for atrial natriuretic factor, β-myosin heavy chain, and α-skeletal actin in left ventricles were all significantly increased in the Cu- deficient hearts. Furthermore, Cu deficiency activated the expression of the c-myc oncogene in the left ventricle. This study thus demonstrated that a molecular program of alterations in embryonic genes, similar to that shown in the work- overloaded heart, was activated in the hypertrophied heart induced by Cu deficiency.

Original languageEnglish (US)
Pages (from-to)282-287
Number of pages6
JournalProceedings of the Society for Experimental Biology and Medicine
Volume223
Issue number3
DOIs
StatePublished - Jan 1 2000
Externally publishedYes

Fingerprint

Gene expression
Copper
Gene Expression
Nutrition
Diet
Dams
Heart Ventricles
Weights and Measures
myc Genes
Ceruloplasmin
Myosin Heavy Chains
Liver
Hypertrophic Cardiomyopathy
Atrial Natriuretic Factor
Actins
Rodentia
Genes
Body Weight
Parturition
Messenger RNA

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Alterations in hypertrophic gene expression by dietary copper restriction in mouse heart. / Kang, Yujian; Wu, Huiyun; Saari, Jack T.

In: Proceedings of the Society for Experimental Biology and Medicine, Vol. 223, No. 3, 01.01.2000, p. 282-287.

Research output: Contribution to journalArticle

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