Alterations in intracellular calcium chelation reproduce developmental differences in repetitive firing and afterhyperpolarizations in rat neocortical neurons

N. M. Lorenzon, Robert Foehring

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Abstract

Many 1-week-old rat sensorimotor cortical neurons exhibit extreme spike-frequency adaptation (neurons only fire for the first 100-250 ms of a 1 s current injection) accompanied by a large, prolonged afterhyperpolarization (AHP). Relatively greater expression of a Ca-dependent K+ current appears to underlie the extreme adaptation observed in immature cells. In the present study, we examined whether altering intracellular Ca2+ buffering by introducing Ca2+ chelators via the recording electrode could reproduce the age-related differences in firing and AHPs. We studied firing behavior and AHPs in 1-week-old and adult neocortical neurons with sharp microelectrodes, under three recording conditions: no chelator, 2 mM BAPTA, or 100-200 mM BAPTA. Our principal findings in regard to firing behavior and AHPs were that (1) adult-low BAPTA neurons mimicked 1 week-control cells, (2) 1 week-high BAPTA neurons were similar to adult-control cells, (3) a greater percentage of 1 week-low BAPTA neurons showed complete adaptation, and (4) adult neurons impaled with high BAPTA electrodes fired in a burst-spiking mode. These data suggest that Ca2+ regulation is qualitatively different in immature and adult neurons.

Original languageEnglish (US)
Pages (from-to)192-203
Number of pages12
JournalDevelopmental Brain Research
Volume84
Issue number2
DOIs
StatePublished - Feb 16 1995

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Calcium
Neurons
Chelating Agents
Electrodes
Microelectrodes
1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid
Injections

All Science Journal Classification (ASJC) codes

  • Developmental Biology
  • Developmental Neuroscience

Cite this

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title = "Alterations in intracellular calcium chelation reproduce developmental differences in repetitive firing and afterhyperpolarizations in rat neocortical neurons",
abstract = "Many 1-week-old rat sensorimotor cortical neurons exhibit extreme spike-frequency adaptation (neurons only fire for the first 100-250 ms of a 1 s current injection) accompanied by a large, prolonged afterhyperpolarization (AHP). Relatively greater expression of a Ca-dependent K+ current appears to underlie the extreme adaptation observed in immature cells. In the present study, we examined whether altering intracellular Ca2+ buffering by introducing Ca2+ chelators via the recording electrode could reproduce the age-related differences in firing and AHPs. We studied firing behavior and AHPs in 1-week-old and adult neocortical neurons with sharp microelectrodes, under three recording conditions: no chelator, 2 mM BAPTA, or 100-200 mM BAPTA. Our principal findings in regard to firing behavior and AHPs were that (1) adult-low BAPTA neurons mimicked 1 week-control cells, (2) 1 week-high BAPTA neurons were similar to adult-control cells, (3) a greater percentage of 1 week-low BAPTA neurons showed complete adaptation, and (4) adult neurons impaled with high BAPTA electrodes fired in a burst-spiking mode. These data suggest that Ca2+ regulation is qualitatively different in immature and adult neurons.",
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