Alterations of the glutathione redox cycle status in fumonisin B1-treated pig kidney cells

Yujian Kang, Judith M. Alexander

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Fumonisin B1 (FB1) causes equine leukoencephalomalacia, porcine pulmonary edema, and liver tumors and chronic nephritis in rats. To investigate mechanisms by which FB1 induces toxicity, effects of FB1 on cellular glutathione (GSH) redox status and GSH depletion on FB1 toxicity in pig kidney (LLC-PK1) cells were studied. Treatment of LLC-PK1 cells with 50 μM FB1 for 24 hours significantly decreased cellular GSH contents from 56 ± 3.2 to 42.7 ± 4.4 nmol/mg protein (p < 0.05) and increased the activities of glutathione reductase (GR) from 25.7 ± 2.4 to 35.7 ± 5.0 μmol NADPH/mg protein (p < 0.05). The activities of glutathione peroxidase (GSHpx), catalase, and Cu,Zn-superoxide dismutase (SOD) were not changed by this treatment. Treatment of LLC-PK1 cells for 12 hours with 0.1 mM buthionine sulf oximine (BSO), a selective inhibitor of the enzyme γ-glutamylcysteine synthetase that catalyzes the rate-limiting reaction in de novo GSH synthesis, decreased cellular GSH levels to about 20% of that found in the control cells. The cells pretreated with 0.1 mM BSO for 12 hours were significantly sensitized to the FB1 cytotoxicity as determined by a long-term survival assay (p < 0.05). The results demonstrate that FB1 changes GSH redox cycle status in LLC-PK1 cells, and GSH may play a role in cytoprotection against FB1 toxicity.

Original languageEnglish (US)
Pages (from-to)121-126
Number of pages6
JournalJournal of Biochemical Toxicology
Volume11
Issue number3
DOIs
StatePublished - Jan 1 1996
Externally publishedYes

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Oxidation-Reduction
Glutathione
Swine
LLC-PK1 Cells
Kidney
Toxicity
Glutamate-Cysteine Ligase
Cytoprotection
fumonisin B1
Glutathione Reductase
Nephritis
Enzyme Inhibitors
Pulmonary Edema
Cytotoxicity
Glutathione Peroxidase
NADP
Liver
Catalase
Horses
Rats

All Science Journal Classification (ASJC) codes

  • Toxicology

Cite this

Alterations of the glutathione redox cycle status in fumonisin B1-treated pig kidney cells. / Kang, Yujian; Alexander, Judith M.

In: Journal of Biochemical Toxicology, Vol. 11, No. 3, 01.01.1996, p. 121-126.

Research output: Contribution to journalArticle

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