Altered actin and myosin expression in muscle during exposure to microgravity

Donald Thomason, P. R. Morrison, V. Oganov, E. Ilyina-Kakueva, F. W. Booth, K. M. Baldwin

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

The mechanism for cardiovascular deconditioning and skeletal muscle atrophy during microgravity is not known. The purpose of the present study was to determine whether a decrease in contractile protein gene expression in the muscle of rats occurred after 14 days of microgravity. No differences existed in the profile of myosin protein isoforms or β-myosin heavy chain mRNA in hearts between the flight and synchronous control groups. On the other hand, differences in the expression of β-myosin heavy chain mRNA relative to the 18S and 28S rRNA in the heart between flight and synchronous control groups were noted with a covariance mapping analysis. Both the vastus intermedius and lateral gastrocnemius muscles exhibited significant (P < 0.05) decreases in skeletal α-actin mRNA per unit of extractable RNA in the flight group compared with the synchronous control group. However, no significant difference for skeletal α-actin mRNA occurred in the triceps brachii muscle between these groups. Cytochrome c mRNA per unit of extractable RNA decreased (P < 0.05) only in the vastus intermedius but not in the lateral gastrocnemius or triceps brachii muscles. In summary, changes in the pretranslational regulation of contractile protein gene expression occur in both heart and skeletal muscle after 14 days of microgravity.

Original languageEnglish (US)
JournalJournal of Applied Physiology
Volume73
Issue number2 SUPPL.
StatePublished - 1992
Externally publishedYes

Fingerprint

Weightlessness
Myosins
Actins
Muscles
Messenger RNA
Contractile Proteins
Skeletal Muscle
Myosin Heavy Chains
Quadriceps Muscle
Control Groups
Cardiovascular Deconditioning
RNA
Gene Expression
Muscular Atrophy
Cytochromes c
Myocardium
Protein Isoforms

All Science Journal Classification (ASJC) codes

  • Endocrinology
  • Physiology
  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

Cite this

Thomason, D., Morrison, P. R., Oganov, V., Ilyina-Kakueva, E., Booth, F. W., & Baldwin, K. M. (1992). Altered actin and myosin expression in muscle during exposure to microgravity. Journal of Applied Physiology, 73(2 SUPPL.).

Altered actin and myosin expression in muscle during exposure to microgravity. / Thomason, Donald; Morrison, P. R.; Oganov, V.; Ilyina-Kakueva, E.; Booth, F. W.; Baldwin, K. M.

In: Journal of Applied Physiology, Vol. 73, No. 2 SUPPL., 1992.

Research output: Contribution to journalArticle

Thomason, D, Morrison, PR, Oganov, V, Ilyina-Kakueva, E, Booth, FW & Baldwin, KM 1992, 'Altered actin and myosin expression in muscle during exposure to microgravity', Journal of Applied Physiology, vol. 73, no. 2 SUPPL..
Thomason D, Morrison PR, Oganov V, Ilyina-Kakueva E, Booth FW, Baldwin KM. Altered actin and myosin expression in muscle during exposure to microgravity. Journal of Applied Physiology. 1992;73(2 SUPPL.).
Thomason, Donald ; Morrison, P. R. ; Oganov, V. ; Ilyina-Kakueva, E. ; Booth, F. W. ; Baldwin, K. M. / Altered actin and myosin expression in muscle during exposure to microgravity. In: Journal of Applied Physiology. 1992 ; Vol. 73, No. 2 SUPPL.
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