Altered Epithelial Density and Expansion of Bulbar Projections of a Discrete HSP70 Immunoreactive Subpopulation of Rat Olfactory Receptor Neurons in Reconstituting Olfactory Epithelium following Exposure to Methyl Bromide

Virginia Mc Millan Carr, George Ring, Steven Youngentob, James E. Schwob, Albert I. Farbman

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

A previously described subpopulation of rat olfactory receptor neurons, the 2A4(+)ORNs, is 1) distinguished by intense constitutive cytoplasmic immunoreactivity to antibodies to the 70-kD heat shock protein (HSP70); 2) occurs sparsely but consistently through ventral and lateral olfactory epithelium (OE); and 3) projects to just two to three consistently located glomeruli in each olfactory bulb (OB) (Carr et al. [1994] J Comp Neurol 348:150-160). Immunoreactivity appears not to be stress-related. To examine the persistence of these features following destruction and reconstitution of the OE, rats were subjected to methyl bromide-induced OE lesion (Schwob et al. [1995] J Comp Neurol 59:15-37; Schwob et al. [1999] J Comp Neurol 412:439-457] and their OE and OBs examined with antibodies to HSP70 6-10.5 weeks postlesion. Lesioned OE showed significantly increased 2A4(+)ORN densities but no alteration of 2A4(+)ORN zonal distribution. The OBs of lesioned animals showed marked expansions of 2A4(+)ORN bulbar projections, with 2-15-fold increases in numbers of glomeruli showing 2A4(+)axons, and projection expansions were greater in animals maintained on chronic food restriction prior to lesioning. Examination of archival 5-month post-MeBr lesion material indicates that altered projection patterns are maintained.

Original languageEnglish (US)
Pages (from-to)475-493
Number of pages19
JournalJournal of Comparative Neurology
Volume469
Issue number4
DOIs
StatePublished - Feb 16 2004
Externally publishedYes

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methyl bromide
Olfactory Receptor Neurons
Olfactory Mucosa
HSP70 Heat-Shock Proteins
Antibodies
Olfactory Bulb
Axons
Food

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

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title = "Altered Epithelial Density and Expansion of Bulbar Projections of a Discrete HSP70 Immunoreactive Subpopulation of Rat Olfactory Receptor Neurons in Reconstituting Olfactory Epithelium following Exposure to Methyl Bromide",
abstract = "A previously described subpopulation of rat olfactory receptor neurons, the 2A4(+)ORNs, is 1) distinguished by intense constitutive cytoplasmic immunoreactivity to antibodies to the 70-kD heat shock protein (HSP70); 2) occurs sparsely but consistently through ventral and lateral olfactory epithelium (OE); and 3) projects to just two to three consistently located glomeruli in each olfactory bulb (OB) (Carr et al. [1994] J Comp Neurol 348:150-160). Immunoreactivity appears not to be stress-related. To examine the persistence of these features following destruction and reconstitution of the OE, rats were subjected to methyl bromide-induced OE lesion (Schwob et al. [1995] J Comp Neurol 59:15-37; Schwob et al. [1999] J Comp Neurol 412:439-457] and their OE and OBs examined with antibodies to HSP70 6-10.5 weeks postlesion. Lesioned OE showed significantly increased 2A4(+)ORN densities but no alteration of 2A4(+)ORN zonal distribution. The OBs of lesioned animals showed marked expansions of 2A4(+)ORN bulbar projections, with 2-15-fold increases in numbers of glomeruli showing 2A4(+)axons, and projection expansions were greater in animals maintained on chronic food restriction prior to lesioning. Examination of archival 5-month post-MeBr lesion material indicates that altered projection patterns are maintained.",
author = "Carr, {Virginia Mc Millan} and George Ring and Steven Youngentob and Schwob, {James E.} and Farbman, {Albert I.}",
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T1 - Altered Epithelial Density and Expansion of Bulbar Projections of a Discrete HSP70 Immunoreactive Subpopulation of Rat Olfactory Receptor Neurons in Reconstituting Olfactory Epithelium following Exposure to Methyl Bromide

AU - Carr, Virginia Mc Millan

AU - Ring, George

AU - Youngentob, Steven

AU - Schwob, James E.

AU - Farbman, Albert I.

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N2 - A previously described subpopulation of rat olfactory receptor neurons, the 2A4(+)ORNs, is 1) distinguished by intense constitutive cytoplasmic immunoreactivity to antibodies to the 70-kD heat shock protein (HSP70); 2) occurs sparsely but consistently through ventral and lateral olfactory epithelium (OE); and 3) projects to just two to three consistently located glomeruli in each olfactory bulb (OB) (Carr et al. [1994] J Comp Neurol 348:150-160). Immunoreactivity appears not to be stress-related. To examine the persistence of these features following destruction and reconstitution of the OE, rats were subjected to methyl bromide-induced OE lesion (Schwob et al. [1995] J Comp Neurol 59:15-37; Schwob et al. [1999] J Comp Neurol 412:439-457] and their OE and OBs examined with antibodies to HSP70 6-10.5 weeks postlesion. Lesioned OE showed significantly increased 2A4(+)ORN densities but no alteration of 2A4(+)ORN zonal distribution. The OBs of lesioned animals showed marked expansions of 2A4(+)ORN bulbar projections, with 2-15-fold increases in numbers of glomeruli showing 2A4(+)axons, and projection expansions were greater in animals maintained on chronic food restriction prior to lesioning. Examination of archival 5-month post-MeBr lesion material indicates that altered projection patterns are maintained.

AB - A previously described subpopulation of rat olfactory receptor neurons, the 2A4(+)ORNs, is 1) distinguished by intense constitutive cytoplasmic immunoreactivity to antibodies to the 70-kD heat shock protein (HSP70); 2) occurs sparsely but consistently through ventral and lateral olfactory epithelium (OE); and 3) projects to just two to three consistently located glomeruli in each olfactory bulb (OB) (Carr et al. [1994] J Comp Neurol 348:150-160). Immunoreactivity appears not to be stress-related. To examine the persistence of these features following destruction and reconstitution of the OE, rats were subjected to methyl bromide-induced OE lesion (Schwob et al. [1995] J Comp Neurol 59:15-37; Schwob et al. [1999] J Comp Neurol 412:439-457] and their OE and OBs examined with antibodies to HSP70 6-10.5 weeks postlesion. Lesioned OE showed significantly increased 2A4(+)ORN densities but no alteration of 2A4(+)ORN zonal distribution. The OBs of lesioned animals showed marked expansions of 2A4(+)ORN bulbar projections, with 2-15-fold increases in numbers of glomeruli showing 2A4(+)axons, and projection expansions were greater in animals maintained on chronic food restriction prior to lesioning. Examination of archival 5-month post-MeBr lesion material indicates that altered projection patterns are maintained.

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