Altered transcriptome signature of phenotypically normal skin fibroblasts heterozygous for CDKN2A in familial melanoma: Relevance to early intervention

Meiyun Fan, Susan R. Pfeffer, Henry T. Lynch, Pamela Cassidy, Sancy Leachman, Lawrence Pfeffer, Levy Kopelovich

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Familial melanoma (FM) is a dominantly heritable cancer that is associated with mutations in the tumor suppressor CDKN2A/p16. In FM, a single inherited "hit" occurs in every somatic cell, enabling interrogation of cultured normal skin fibroblasts (SFs) from FM gene carriers as surrogates for the cell of tumor origin, namely the melanocyte.We compared the gene expression profile of SFs from FM individuals with two distinct CDKN2A/p16 mutations (V126D-p16 and R87P-p16) with the gene expression profile of SFs from age-matched individuals without p16 mutations and with no family history of melanoma. We show an altered transcriptome signature in normal SFs bearing a single-hit inherited mutation in the CDKN2A/p16 gene, wherein some of these abnormalalterations recapitulate changes observed in the corresponding cancer.Significantly, the extent of the alterations is mutation-site specific with the R87P-p16 mutation being more disruptive than the V126D-p16 mutation. We also examined changes in gene expression after exposure to ultraviolet (UV) radiation to define potential early biomarkers triggered by sun exposure. UV treatment of SFs from FM families induces distinct alterations in genes related to cell cycle regulation andDNA damage responses that are also reported to be dysregulated in melanoma. Importantly, these changes were diametrically opposed to UV-induced changes in SF from normal controls. We posit that changes identified in the transcriptome of SF from FM mutation carriers represent early events critical for melanoma development. As such, they may serve as specific biomarkers of increased risk as well as molecular targets for personalized prevention strategies in high-risk populations.

Original languageEnglish (US)
Pages (from-to)128-141
Number of pages14
JournalOncotarget
Volume4
Issue number1
DOIs
StatePublished - Jan 1 2013

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Transcriptome
Fibroblasts
Skin
Mutation
p16 Genes
Melanoma
Neoplasms
Biomarkers
Cutaneous Malignant Melanoma
Melanocytes
Solar System
Genes
Cell Cycle
Radiation
Gene Expression
Population

All Science Journal Classification (ASJC) codes

  • Oncology

Cite this

Altered transcriptome signature of phenotypically normal skin fibroblasts heterozygous for CDKN2A in familial melanoma : Relevance to early intervention. / Fan, Meiyun; Pfeffer, Susan R.; Lynch, Henry T.; Cassidy, Pamela; Leachman, Sancy; Pfeffer, Lawrence; Kopelovich, Levy.

In: Oncotarget, Vol. 4, No. 1, 01.01.2013, p. 128-141.

Research output: Contribution to journalArticle

Fan, Meiyun ; Pfeffer, Susan R. ; Lynch, Henry T. ; Cassidy, Pamela ; Leachman, Sancy ; Pfeffer, Lawrence ; Kopelovich, Levy. / Altered transcriptome signature of phenotypically normal skin fibroblasts heterozygous for CDKN2A in familial melanoma : Relevance to early intervention. In: Oncotarget. 2013 ; Vol. 4, No. 1. pp. 128-141.
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