Alternatively spliced Robo2 isoforms in zebrafish and rat

Ertugrul Dalkic, Cem Kuscu, Ceren Sucularli, Iraz T. Aydin, Kamil C. Akcali, Ozlen Konu

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Robo2, a member of the robo gene family, functions as a repulsive axon guidance receptor as well as a regulator of cell migration and tissue morphogenesis in different taxa. In this study, a novel isoform of the zebrafish robo2 (robo2_tv2), which included an otherwise alternatively spliced exon (CAE), has been characterized. Robo2_tv2 is expressed differentially in most non-neuronal tissues of adult zebrafish whereas robo2_tv1 expression to a great extent is restricted to the brain and eye. In zebrafish, robo2_tv2 exhibits a very-low-level basal expression starting from 1 day post fertilization until the mid-larval stages, at which time its expression increases dramatically and could be detected throughout adulthood. Our findings demonstrate that the amino acid sequence coded by CAE of the robo2 gene is highly conserved between zebrafish and mammals, and also contains conserved motifs shared with robo1 and robo4 but not with robo3. Furthermore, we provide an account of differential transcription of the CAE homolog in various tissues of the adult rat. These results suggest that the alternatively spliced robo2 isoforms may exhibit tissue specificity.

Original languageEnglish (US)
Pages (from-to)555-563
Number of pages9
JournalDevelopment Genes and Evolution
Volume216
Issue number9
DOIs
StatePublished - Sep 1 2006

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Zebrafish
Protein Isoforms
Organ Specificity
Morphogenesis
Fertilization
Genes
Cell Movement
Mammals
Amino Acid Sequence
Exons
Brain

All Science Journal Classification (ASJC) codes

  • Genetics
  • Developmental Biology

Cite this

Dalkic, E., Kuscu, C., Sucularli, C., Aydin, I. T., Akcali, K. C., & Konu, O. (2006). Alternatively spliced Robo2 isoforms in zebrafish and rat. Development Genes and Evolution, 216(9), 555-563. https://doi.org/10.1007/s00427-006-0070-y

Alternatively spliced Robo2 isoforms in zebrafish and rat. / Dalkic, Ertugrul; Kuscu, Cem; Sucularli, Ceren; Aydin, Iraz T.; Akcali, Kamil C.; Konu, Ozlen.

In: Development Genes and Evolution, Vol. 216, No. 9, 01.09.2006, p. 555-563.

Research output: Contribution to journalArticle

Dalkic, E, Kuscu, C, Sucularli, C, Aydin, IT, Akcali, KC & Konu, O 2006, 'Alternatively spliced Robo2 isoforms in zebrafish and rat', Development Genes and Evolution, vol. 216, no. 9, pp. 555-563. https://doi.org/10.1007/s00427-006-0070-y
Dalkic, Ertugrul ; Kuscu, Cem ; Sucularli, Ceren ; Aydin, Iraz T. ; Akcali, Kamil C. ; Konu, Ozlen. / Alternatively spliced Robo2 isoforms in zebrafish and rat. In: Development Genes and Evolution. 2006 ; Vol. 216, No. 9. pp. 555-563.
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