Amelin

A 4.1-related spectrin-binding protein found in neuronal cell bodies and dendrites

K. E. Krebs, I. S. Zagon, Steven Goodman

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

An immunoreactive, structural, and functional analog of erythrocyte protein 4.1 is present in neuronal cell bodies and dendrites. Other investigators have described the isolation of a 4.1 analog in brain with structural characteristics suggesting that its identity was synapsin I, a neuronal phosphoprotein localized in the presynaptic terminal in association with small synaptic vesicles. In this report we demonstrate that the cell body/dendritic form of brain protein 4.1, which we have named amelin, is distinct from that of synapsin I on the basis of subcellular localization, migration in 2-dimensional gel electrophoresis, and structural criteria. We also demonstrate that amelin, like synapsin I, can bind brain spectrin on nitrocellulose paper. Neither amelin nor synapsin I binds calmodulin, as determined by a blot binding assay. We hypothesize that there exists in brain a family of 4.1-related proteins with distinct subcellular localization and function.

Original languageEnglish (US)
Pages (from-to)3907-3914
Number of pages8
JournalJournal of Neuroscience
Volume7
Issue number12
StatePublished - Dec 1 1987
Externally publishedYes

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Synapsins
Dendrites
Brain
Spectrin
Proteins
Collodion
Synaptic Vesicles
Phosphoproteins
Presynaptic Terminals
Calmodulin
Electrophoresis
Erythrocytes
Gels
Research Personnel
Cell Body
spectrin-binding proteins

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

Amelin : A 4.1-related spectrin-binding protein found in neuronal cell bodies and dendrites. / Krebs, K. E.; Zagon, I. S.; Goodman, Steven.

In: Journal of Neuroscience, Vol. 7, No. 12, 01.12.1987, p. 3907-3914.

Research output: Contribution to journalArticle

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