Amidase, a cell wall hydrolase, elicits protective immunity against Staphylococcus aureus and S. epidermidis

Nisha Nair, Vivek Vinod, Maneesha K. Suresh, Sukhithasri Vijayrajratnam, Lalitha Biswas, Reshmi Peethambaran, Anil Kumar Vasudevan, Raja Biswas

Research output: Contribution to journalArticle

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Abstract

The morbidity and the mortality associated with Staphylococcus aureus and S. epidermidis infections have greatly increased due to the rapid emergence of highly virulent and antibiotic resistant strains. Development of a vaccine-based therapy is greatly desired. However, no staphylococcal vaccine is available till date. In this study, we have identified Major amidase (Atl-AM) as a prime candidate for future vaccine design against these pathogens. Atl-AM is a multi-functional non-covalently cell wall associated protein which is involved in staphylococcal cell separation after cell division, host extracellular matrix adhesion and biofilm formation. Atl-AM is present on the surface of diverse S. aureus and S. epidermidis strains. When used in combination with Freund's adjuvant, Atl-AM generated a mixed Th1 and Th2 mediated immune response which is skewed more toward Th1; and showed increased production of opsonophagocytic IgG2a and IgG2b antibodies. Significant protective immune response was observed when vaccinated mice were challenged with S. aureus or S. epidermidis. Vaccination prevented the systemic dissemination of both organisms. Our results demonstrate the remarkable efficacy of Atl-AM as a vaccine candidate against both of these pathogens.

Original languageEnglish (US)
Pages (from-to)314-321
Number of pages8
JournalInternational Journal of Biological Macromolecules
Volume77
DOIs
StatePublished - Jun 1 2015

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amidase
Hydrolases
Staphylococcus aureus
Immunity
Vaccines
Cells
Pathogens
Staphylococcal Vaccines
Active Immunotherapy
Freund's Adjuvant
Cell Separation
Biofilms
Cell Division
Cell Wall
Extracellular Matrix
Vaccination
Adhesion
Anti-Bacterial Agents
Morbidity
Mortality

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Biochemistry
  • Molecular Biology

Cite this

Amidase, a cell wall hydrolase, elicits protective immunity against Staphylococcus aureus and S. epidermidis. / Nair, Nisha; Vinod, Vivek; Suresh, Maneesha K.; Vijayrajratnam, Sukhithasri; Biswas, Lalitha; Peethambaran, Reshmi; Vasudevan, Anil Kumar; Biswas, Raja.

In: International Journal of Biological Macromolecules, Vol. 77, 01.06.2015, p. 314-321.

Research output: Contribution to journalArticle

Nair, N, Vinod, V, Suresh, MK, Vijayrajratnam, S, Biswas, L, Peethambaran, R, Vasudevan, AK & Biswas, R 2015, 'Amidase, a cell wall hydrolase, elicits protective immunity against Staphylococcus aureus and S. epidermidis', International Journal of Biological Macromolecules, vol. 77, pp. 314-321. https://doi.org/10.1016/j.ijbiomac.2015.03.047
Nair, Nisha ; Vinod, Vivek ; Suresh, Maneesha K. ; Vijayrajratnam, Sukhithasri ; Biswas, Lalitha ; Peethambaran, Reshmi ; Vasudevan, Anil Kumar ; Biswas, Raja. / Amidase, a cell wall hydrolase, elicits protective immunity against Staphylococcus aureus and S. epidermidis. In: International Journal of Biological Macromolecules. 2015 ; Vol. 77. pp. 314-321.
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