Amphotericin B induces expression of genes encoding chemokines and cell adhesion molecules in the human monocytic cell line THP-1

Phillip Rogers, Jonathan K. Stiles, Stanley W. Chapman, John D. Cleary

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Amphotericin B is known to elicit immunomodulatory effects on neutrophil, monocyte, and lymphocyte function. It also has been shown to induce the release of proinflammatory cytokines from human monocytes and macrophages. Release of these cytokines has been associated with the infusion-related toxicity observed after administration of this drug. The present study demonstrates that amphotericin B increases mRNA for the chemokines interleukin (IL)-8, monocyte chemoattractant protein (MCP)-1, and macrophage inflammatory protein (MIP)-1β, as well as the cell adhesion molecules intercellular adhesion molecule (ICAM)-1 and CD44 in the human monocytic cell line THP-1. Amphotericin B increased the concentrations of IL-8, MCP-1, and MIP-1β in a dose-dependent fashion. Amphotericin B also induced expression of ICAM-1 but not CD44 in these cells. Production of these proteins in response to amphotericin B may play a role in the immunomodulatory activity and toxicity of this antifungal agent.

Original languageEnglish (US)
Pages (from-to)1280-1283
Number of pages4
JournalJournal of Infectious Diseases
Volume182
Issue number4
DOIs
StatePublished - Jan 1 2000
Externally publishedYes

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Cell Adhesion Molecules
Amphotericin B
Chemokines
Gene Expression
Cell Line
Macrophage Inflammatory Proteins
Chemokine CCL2
Intercellular Adhesion Molecule-1
Interleukin-8
Monocytes
Cytokines
Antifungal Agents
Neutrophils
Macrophages
Lymphocytes
Messenger RNA
Pharmaceutical Preparations
Proteins

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Infectious Diseases

Cite this

Amphotericin B induces expression of genes encoding chemokines and cell adhesion molecules in the human monocytic cell line THP-1. / Rogers, Phillip; Stiles, Jonathan K.; Chapman, Stanley W.; Cleary, John D.

In: Journal of Infectious Diseases, Vol. 182, No. 4, 01.01.2000, p. 1280-1283.

Research output: Contribution to journalArticle

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