An analysis of total RNA translation products of rat liver during regeneration with a comparison to fetal liver

H. Stephen Beyer, Burt Sharp, Leslie Zieve

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

We explored differences in the mRNA populations of regenerating, sham-operated, and fetal rat liver using two-dimensional gel electrophoresis to resolve the radiolabeled protein products of liver total RNA translated in vitro. Twenty-four translation products were changed significantly after partial hepatectomy and sham hepatectomy. Nine of the 24 products changed after partial hepatectomy only, while the remainder also changed after sham hepatectomy. Two of the nine increased during regeneration to relative levels similar to those found in fetal liver. However, substantial differences also exist between the translation products of regenerating and fetal liver. These results suggest that the response to partial hepatectomy involves the alteration of mRNAs present in normal liver and that this response does not duplicate the fetal pattern of hepatic total RNA translation activity.

Original languageEnglish (US)
Pages (from-to)119-128
Number of pages10
JournalCell Differentiation and Development
Volume28
Issue number2
DOIs
StatePublished - Jan 1 1989

Fingerprint

Liver Regeneration
Hepatectomy
RNA
Liver
Messenger RNA
Electrophoresis, Gel, Two-Dimensional
Regeneration
Population
Proteins

All Science Journal Classification (ASJC) codes

  • Developmental Biology

Cite this

An analysis of total RNA translation products of rat liver during regeneration with a comparison to fetal liver. / Beyer, H. Stephen; Sharp, Burt; Zieve, Leslie.

In: Cell Differentiation and Development, Vol. 28, No. 2, 01.01.1989, p. 119-128.

Research output: Contribution to journalArticle

@article{e1ce0fb3abc24fdc958aba0b700cb546,
title = "An analysis of total RNA translation products of rat liver during regeneration with a comparison to fetal liver",
abstract = "We explored differences in the mRNA populations of regenerating, sham-operated, and fetal rat liver using two-dimensional gel electrophoresis to resolve the radiolabeled protein products of liver total RNA translated in vitro. Twenty-four translation products were changed significantly after partial hepatectomy and sham hepatectomy. Nine of the 24 products changed after partial hepatectomy only, while the remainder also changed after sham hepatectomy. Two of the nine increased during regeneration to relative levels similar to those found in fetal liver. However, substantial differences also exist between the translation products of regenerating and fetal liver. These results suggest that the response to partial hepatectomy involves the alteration of mRNAs present in normal liver and that this response does not duplicate the fetal pattern of hepatic total RNA translation activity.",
author = "Beyer, {H. Stephen} and Burt Sharp and Leslie Zieve",
year = "1989",
month = "1",
day = "1",
doi = "10.1016/0922-3371(89)90048-8",
language = "English (US)",
volume = "28",
pages = "119--128",
journal = "Mechanisms of Development",
issn = "0925-4773",
publisher = "Elsevier Ireland Ltd",
number = "2",

}

TY - JOUR

T1 - An analysis of total RNA translation products of rat liver during regeneration with a comparison to fetal liver

AU - Beyer, H. Stephen

AU - Sharp, Burt

AU - Zieve, Leslie

PY - 1989/1/1

Y1 - 1989/1/1

N2 - We explored differences in the mRNA populations of regenerating, sham-operated, and fetal rat liver using two-dimensional gel electrophoresis to resolve the radiolabeled protein products of liver total RNA translated in vitro. Twenty-four translation products were changed significantly after partial hepatectomy and sham hepatectomy. Nine of the 24 products changed after partial hepatectomy only, while the remainder also changed after sham hepatectomy. Two of the nine increased during regeneration to relative levels similar to those found in fetal liver. However, substantial differences also exist between the translation products of regenerating and fetal liver. These results suggest that the response to partial hepatectomy involves the alteration of mRNAs present in normal liver and that this response does not duplicate the fetal pattern of hepatic total RNA translation activity.

AB - We explored differences in the mRNA populations of regenerating, sham-operated, and fetal rat liver using two-dimensional gel electrophoresis to resolve the radiolabeled protein products of liver total RNA translated in vitro. Twenty-four translation products were changed significantly after partial hepatectomy and sham hepatectomy. Nine of the 24 products changed after partial hepatectomy only, while the remainder also changed after sham hepatectomy. Two of the nine increased during regeneration to relative levels similar to those found in fetal liver. However, substantial differences also exist between the translation products of regenerating and fetal liver. These results suggest that the response to partial hepatectomy involves the alteration of mRNAs present in normal liver and that this response does not duplicate the fetal pattern of hepatic total RNA translation activity.

UR - http://www.scopus.com/inward/record.url?scp=0024355518&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024355518&partnerID=8YFLogxK

U2 - 10.1016/0922-3371(89)90048-8

DO - 10.1016/0922-3371(89)90048-8

M3 - Article

VL - 28

SP - 119

EP - 128

JO - Mechanisms of Development

JF - Mechanisms of Development

SN - 0925-4773

IS - 2

ER -