An AOTF-based dual-modality hyperspectral imaging system (DMHSI) capable of simultaneous fluorescence and reflectance imaging

Matt E. Martin, Musundi Wabuyele, Masoud Panjehpour, Bergein Overholt, Robert Denovo, Stephen Kennel, Glenn Cunningham, Tuan Vo-Dinh

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

An acousto-optic tunable filter (AOTF)-based system for dual-modality hyperspectral imaging (DMHSI) has been developed for use in characterization of normal and malignant mouse tissue. The system consists of a laser, endoscope, AOTF, and two cameras coupled with optics and electronics. Initial results show that the system can delineate normal and malignant mouse tissues real-time. The analysis shows that malignant tissues consistently exhibit less fluorescent intensity in the wavelength band from 440 to 540 nm with a peak intensity of around 490 nm. The analysis also shows key spectroscopic differences between normal and malignant tissues. Further, these results are compared to real-time spectroscopic data and show good correlation.

Original languageEnglish (US)
Pages (from-to)149-155
Number of pages7
JournalMedical Engineering and Physics
Volume28
Issue number2
DOIs
StatePublished - Mar 1 2006

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Optical Imaging
Imaging systems
Optics
Fluorescence
Tissue
Imaging techniques
Endoscopy
Endoscopes
Lasers
Electronic equipment
Cameras
Wavelength
Hyperspectral imaging

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biomedical Engineering

Cite this

An AOTF-based dual-modality hyperspectral imaging system (DMHSI) capable of simultaneous fluorescence and reflectance imaging. / Martin, Matt E.; Wabuyele, Musundi; Panjehpour, Masoud; Overholt, Bergein; Denovo, Robert; Kennel, Stephen; Cunningham, Glenn; Vo-Dinh, Tuan.

In: Medical Engineering and Physics, Vol. 28, No. 2, 01.03.2006, p. 149-155.

Research output: Contribution to journalArticle

Martin, Matt E. ; Wabuyele, Musundi ; Panjehpour, Masoud ; Overholt, Bergein ; Denovo, Robert ; Kennel, Stephen ; Cunningham, Glenn ; Vo-Dinh, Tuan. / An AOTF-based dual-modality hyperspectral imaging system (DMHSI) capable of simultaneous fluorescence and reflectance imaging. In: Medical Engineering and Physics. 2006 ; Vol. 28, No. 2. pp. 149-155.
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