An avian tumor virus promoter directs expression of plasmid genes in Escherichia coli

S. Alex Mitsialis, James F. Young, Peter Palese, Ramareddy Guntaka

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

A sequence in the long terminal repeat (LTR) of avian tumor virus (ATV) DNA was shown to contain a promoter active in Escherichia coli. For this analysis the bacterial promoters for the tetracycline (Tc) and neomycin (Nm) resistance genes were deleted from different plasmids and replaced with various fragments derived from the ATV DNA. Expression of the drug-resistant phenotype in the recombinant plasmids at levels comparable to or greater than those found with parental bacterial promoters was shown to be dependent on the presence of an intact sequence ranging from nucleotide +19 to -23 (relative to the cap site) in the ATV DNA. Comparison of the consensus bacterial promoter with the nucleotide sequence in this region revealed strong similarities.

Original languageEnglish (US)
Pages (from-to)217-225
Number of pages9
JournalGene
Volume16
Issue number1-3
DOIs
StatePublished - Jan 1 1981

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DNA Tumor Viruses
Oncogenic Viruses
Carcinogens
Plasmids
Escherichia coli
Gene Expression
Tetracycline Resistance
Neomycin
Terminal Repeat Sequences
Phenotype
Pharmaceutical Preparations
Genes

All Science Journal Classification (ASJC) codes

  • Medicine(all)
  • Genetics

Cite this

An avian tumor virus promoter directs expression of plasmid genes in Escherichia coli. / Mitsialis, S. Alex; Young, James F.; Palese, Peter; Guntaka, Ramareddy.

In: Gene, Vol. 16, No. 1-3, 01.01.1981, p. 217-225.

Research output: Contribution to journalArticle

Mitsialis, S. Alex ; Young, James F. ; Palese, Peter ; Guntaka, Ramareddy. / An avian tumor virus promoter directs expression of plasmid genes in Escherichia coli. In: Gene. 1981 ; Vol. 16, No. 1-3. pp. 217-225.
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