An immunochemical analysis of a myelin basic protein serum factor

Cross reactivity with residues 69–71 of the rabbit encephalitogenic sequence 65–74 of myelin basic protein

Nicholas Potter, G. A. Hashim, E. D. Day

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

A partially purified myelin basic protein serum factor (MBP‐SF), cross‐reactive with residues 65–74 (TTHYGSLPQK) of myelin basic protein, has been employed in an immunochemical study to identify the nature of the cross‐reacting determinant, more precisely. To probe the structural requirements of this determinant, Scatchard inhibition analyses and competitive peptide inhibition radioimmunoassays were employed with a series of peptide analogs of the 65‐74 region and with three different reagent antisera: a rabbit anti‐rat myelin antiserum (#My05) and two antisera, one rabbit (#162) and one chicken (#66), raised against synthetic peptide S24 (TTHYGSLPQKG). Scatchard inhibition analyses with MBP‐SF revealed specific inhibition of binding of 125I‐S24 to #162 and #My05, but not to #66. Further delineation of the structural requirements of the cross‐reactive determinant, employing a liquid‐phase radioimmunoassay, revealed a unique reactivity pattern for the chicken anti‐S24 antiserum which, unlike #162 and #My05, did not cross‐react under high‐affinity conditions with synthetic peptide S20 (GSLPQK, representing the C‐terminal half of S24). This, in concert with the Scatchard data, is suggestive of the presence of a cross‐reactive determinant centered around residues 69–71 of MBP.

Original languageEnglish (US)
Pages (from-to)457-466
Number of pages10
JournalJournal of Neuroscience Research
Volume15
Issue number4
DOIs
StatePublished - Jan 1 1986
Externally publishedYes

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Myelin Basic Protein
Immune Sera
Rabbits
Peptides
Radioimmunoassay
Chickens
Myelin Sheath
myelin basic protein-serum factors

All Science Journal Classification (ASJC) codes

  • Cellular and Molecular Neuroscience

Cite this

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title = "An immunochemical analysis of a myelin basic protein serum factor: Cross reactivity with residues 69–71 of the rabbit encephalitogenic sequence 65–74 of myelin basic protein",
abstract = "A partially purified myelin basic protein serum factor (MBP‐SF), cross‐reactive with residues 65–74 (TTHYGSLPQK) of myelin basic protein, has been employed in an immunochemical study to identify the nature of the cross‐reacting determinant, more precisely. To probe the structural requirements of this determinant, Scatchard inhibition analyses and competitive peptide inhibition radioimmunoassays were employed with a series of peptide analogs of the 65‐74 region and with three different reagent antisera: a rabbit anti‐rat myelin antiserum (#My05) and two antisera, one rabbit (#162) and one chicken (#66), raised against synthetic peptide S24 (TTHYGSLPQKG). Scatchard inhibition analyses with MBP‐SF revealed specific inhibition of binding of 125I‐S24 to #162 and #My05, but not to #66. Further delineation of the structural requirements of the cross‐reactive determinant, employing a liquid‐phase radioimmunoassay, revealed a unique reactivity pattern for the chicken anti‐S24 antiserum which, unlike #162 and #My05, did not cross‐react under high‐affinity conditions with synthetic peptide S20 (GSLPQK, representing the C‐terminal half of S24). This, in concert with the Scatchard data, is suggestive of the presence of a cross‐reactive determinant centered around residues 69–71 of MBP.",
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AU - Day, E. D.

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