An improved technique for cerebrospinal fluid collection of cisterna magna in Rhesus monkeys

Xianglong Li, Pengfei Han, Yingjia Guo, Huaiqiang Sun, Ying Xiao, Yujian Kang

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Background: Currently-used cerebellomedullary cistern puncture method for collecting cerebrospinal fluid (CSF) from monkeys is simple, inexpensive, and practical, but with high risk for brainstem injury and CSF blood contamination. An improved technique was thus developed and characterized. Method: Magnetic resonance imaging was used to identify the space and position of the cisterna magna in monkeys. Accordingly, a newly defined procedure for needle punctuation was tested in comparison with the traditional method. Blood contamination in CSF samples and brainstem injury were determined to define the superior of the improved method over the transitional method. Results: The cisterna magna in monkeys was found to be a "▿" shape. The needle was punctured into the cisterna magna aiming at the wider superior gap avoided brainstem injury. The improved method showed that the rate of blood contamination in the CSF samples was reduced from 66.7% to 16.7%, the higher rate of blood contamination was associated with higher risk for brainstem injury.Comparison with existing methods: In traditional method, the needle is punctured aiming at the inferior gap with high density of blood vessels. In improved method, the needle is punctured aiming at the superior gap, pointing to the nose root while advancing the needle and avoiding injury to blood vessels. Conclusions: This improved technique not only avoids blood contamination of CSF, but also prevents brainstem injury during the process of CSF collection. It is recommended for adaptation for CSF collection in monkeys.

Original languageEnglish (US)
Pages (from-to)59-65
Number of pages7
JournalJournal of Neuroscience Methods
Volume249
DOIs
StatePublished - Jul 5 2015

Fingerprint

Cisterna Magna
Macaca mulatta
Cerebrospinal Fluid
Brain Stem
Needles
Haplorhini
Wounds and Injuries
Blood Vessels
Nose
Punctures
Magnetic Resonance Imaging

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

An improved technique for cerebrospinal fluid collection of cisterna magna in Rhesus monkeys. / Li, Xianglong; Han, Pengfei; Guo, Yingjia; Sun, Huaiqiang; Xiao, Ying; Kang, Yujian.

In: Journal of Neuroscience Methods, Vol. 249, 05.07.2015, p. 59-65.

Research output: Contribution to journalArticle

Li, Xianglong ; Han, Pengfei ; Guo, Yingjia ; Sun, Huaiqiang ; Xiao, Ying ; Kang, Yujian. / An improved technique for cerebrospinal fluid collection of cisterna magna in Rhesus monkeys. In: Journal of Neuroscience Methods. 2015 ; Vol. 249. pp. 59-65.
@article{2b31f20dc5c44b34869508b0bca009a9,
title = "An improved technique for cerebrospinal fluid collection of cisterna magna in Rhesus monkeys",
abstract = "Background: Currently-used cerebellomedullary cistern puncture method for collecting cerebrospinal fluid (CSF) from monkeys is simple, inexpensive, and practical, but with high risk for brainstem injury and CSF blood contamination. An improved technique was thus developed and characterized. Method: Magnetic resonance imaging was used to identify the space and position of the cisterna magna in monkeys. Accordingly, a newly defined procedure for needle punctuation was tested in comparison with the traditional method. Blood contamination in CSF samples and brainstem injury were determined to define the superior of the improved method over the transitional method. Results: The cisterna magna in monkeys was found to be a {"}▿{"} shape. The needle was punctured into the cisterna magna aiming at the wider superior gap avoided brainstem injury. The improved method showed that the rate of blood contamination in the CSF samples was reduced from 66.7{\%} to 16.7{\%}, the higher rate of blood contamination was associated with higher risk for brainstem injury.Comparison with existing methods: In traditional method, the needle is punctured aiming at the inferior gap with high density of blood vessels. In improved method, the needle is punctured aiming at the superior gap, pointing to the nose root while advancing the needle and avoiding injury to blood vessels. Conclusions: This improved technique not only avoids blood contamination of CSF, but also prevents brainstem injury during the process of CSF collection. It is recommended for adaptation for CSF collection in monkeys.",
author = "Xianglong Li and Pengfei Han and Yingjia Guo and Huaiqiang Sun and Ying Xiao and Yujian Kang",
year = "2015",
month = "7",
day = "5",
doi = "10.1016/j.jneumeth.2015.04.004",
language = "English (US)",
volume = "249",
pages = "59--65",
journal = "Journal of Neuroscience Methods",
issn = "0165-0270",
publisher = "Elsevier",

}

TY - JOUR

T1 - An improved technique for cerebrospinal fluid collection of cisterna magna in Rhesus monkeys

AU - Li, Xianglong

AU - Han, Pengfei

AU - Guo, Yingjia

AU - Sun, Huaiqiang

AU - Xiao, Ying

AU - Kang, Yujian

PY - 2015/7/5

Y1 - 2015/7/5

N2 - Background: Currently-used cerebellomedullary cistern puncture method for collecting cerebrospinal fluid (CSF) from monkeys is simple, inexpensive, and practical, but with high risk for brainstem injury and CSF blood contamination. An improved technique was thus developed and characterized. Method: Magnetic resonance imaging was used to identify the space and position of the cisterna magna in monkeys. Accordingly, a newly defined procedure for needle punctuation was tested in comparison with the traditional method. Blood contamination in CSF samples and brainstem injury were determined to define the superior of the improved method over the transitional method. Results: The cisterna magna in monkeys was found to be a "▿" shape. The needle was punctured into the cisterna magna aiming at the wider superior gap avoided brainstem injury. The improved method showed that the rate of blood contamination in the CSF samples was reduced from 66.7% to 16.7%, the higher rate of blood contamination was associated with higher risk for brainstem injury.Comparison with existing methods: In traditional method, the needle is punctured aiming at the inferior gap with high density of blood vessels. In improved method, the needle is punctured aiming at the superior gap, pointing to the nose root while advancing the needle and avoiding injury to blood vessels. Conclusions: This improved technique not only avoids blood contamination of CSF, but also prevents brainstem injury during the process of CSF collection. It is recommended for adaptation for CSF collection in monkeys.

AB - Background: Currently-used cerebellomedullary cistern puncture method for collecting cerebrospinal fluid (CSF) from monkeys is simple, inexpensive, and practical, but with high risk for brainstem injury and CSF blood contamination. An improved technique was thus developed and characterized. Method: Magnetic resonance imaging was used to identify the space and position of the cisterna magna in monkeys. Accordingly, a newly defined procedure for needle punctuation was tested in comparison with the traditional method. Blood contamination in CSF samples and brainstem injury were determined to define the superior of the improved method over the transitional method. Results: The cisterna magna in monkeys was found to be a "▿" shape. The needle was punctured into the cisterna magna aiming at the wider superior gap avoided brainstem injury. The improved method showed that the rate of blood contamination in the CSF samples was reduced from 66.7% to 16.7%, the higher rate of blood contamination was associated with higher risk for brainstem injury.Comparison with existing methods: In traditional method, the needle is punctured aiming at the inferior gap with high density of blood vessels. In improved method, the needle is punctured aiming at the superior gap, pointing to the nose root while advancing the needle and avoiding injury to blood vessels. Conclusions: This improved technique not only avoids blood contamination of CSF, but also prevents brainstem injury during the process of CSF collection. It is recommended for adaptation for CSF collection in monkeys.

UR - http://www.scopus.com/inward/record.url?scp=84928683498&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84928683498&partnerID=8YFLogxK

U2 - 10.1016/j.jneumeth.2015.04.004

DO - 10.1016/j.jneumeth.2015.04.004

M3 - Article

VL - 249

SP - 59

EP - 65

JO - Journal of Neuroscience Methods

JF - Journal of Neuroscience Methods

SN - 0165-0270

ER -