An insertion of intracisternal A-particle retrotransposon in a novel member of the phosphoglycerate mutase family in the lew allele of mutant mice

Yan Jiao, Xiudong Jin, Jian Yan, Feng Jiao, Xinmin Li, Bruce A. Roe, Harry W. Jarrett, Weikuan Gu

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Intracisternal A-particle retrotransposons (IAPs) are known, moveable, retrovirus-like elements and are defective in envelope protein synthesis in the mouse genome. Insertion of IAP elements can either interupt or enhance gene function or expression. Using a mouse model called lethal wasting (lew), we recently identified the insertion of an IAP sequence in a gene, 9630033F20Rik, that contains domains involved in glycolysis. The expression pattern of the 9630033F20Rik gene between various normal and diseased tissues was determined by semi-quantitative RT-PCR. The effect of the insertion mutation in 9630033F20Rik on glycolysis in heart, muscle, and brain tissues was further investigated using oligonuleotide microarray analysis. Results indicated that the expression of 9630033F20Rik is ubiquitous and its signal is relatively higher in heart and brain tissues. The insertion caused the deletion of exon 5 and decreased expression of this gene in all the tissues studied in the lew mice. Changes in the expression levels of glycolytic genes mainly occured in muscle tissue, raising a possibility that 9630033F20Rik may function as one of the transcriptional regulators of glycolytic genes in skeletal muscle. However, considering the fact that a single nucleotide mutation in vesicle-associated membrane protein 1 (VAMP1) has been reported as the causal gene for the lew mouse, how much of an impact the IAP insertion in the lew mouse phenotype has on glycolytic genes compared to the effect from the VAMP1 mutation responsible for the lew mouse phenotype should be further investigated.

Original languageEnglish (US)
Pages (from-to)327-334
Number of pages8
JournalGenes and Genetic Systems
Volume84
Issue number5
DOIs
StatePublished - Dec 1 2009

Fingerprint

Phosphoglycerate Mutase
Lethal Genes
Retroelements
Vesicle-Associated Membrane Protein 1
Genes
Glycolysis
Intracisternal A-Particle Genes
Phenotype
Muscles
Mutation
Insertional Mutagenesis
Brain
Regulator Genes
Microarray Analysis
Exons
Myocardium
Skeletal Muscle
Nucleotides
Genome
Gene Expression

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

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An insertion of intracisternal A-particle retrotransposon in a novel member of the phosphoglycerate mutase family in the lew allele of mutant mice. / Jiao, Yan; Jin, Xiudong; Yan, Jian; Jiao, Feng; Li, Xinmin; Roe, Bruce A.; Jarrett, Harry W.; Gu, Weikuan.

In: Genes and Genetic Systems, Vol. 84, No. 5, 01.12.2009, p. 327-334.

Research output: Contribution to journalArticle

Jiao, Yan ; Jin, Xiudong ; Yan, Jian ; Jiao, Feng ; Li, Xinmin ; Roe, Bruce A. ; Jarrett, Harry W. ; Gu, Weikuan. / An insertion of intracisternal A-particle retrotransposon in a novel member of the phosphoglycerate mutase family in the lew allele of mutant mice. In: Genes and Genetic Systems. 2009 ; Vol. 84, No. 5. pp. 327-334.
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