An N-methyl-d-aspartate receptor mediated excitatory postsynaptic potential evoked in subthalamic neurons in an in vitro slice preparation of the rat

H. Nakanishi, Hitoshi Kita, S. T. Kitai

Research output: Contribution to journalArticle

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Abstract

Subthalamic (STH) neurons with slow EPSPs mediated by an N-methyl-d-aspartate (NMDA) receptor were studied in rat brain slice preparation. When STH neurons were intracellularly recorded with KC1-filled electrodes, stimulation of the internal capsule (IC) evoked a short duration depolarization followed by a slow excitatory postsynaptic potential (EPSP) lasting 100-200 ms. The amplitude of the slow EPSP was increased when the neuron was hyperpolarized by a low intensity current injection but was blocked when it was hyperpolarized with strong current. The slow EPSP was reversibly suppressed by application of 30-50 μM dl-2-amino-5-phosphonovareric acid (APV). STH neurons also were recorded, with potassium methylsulfate filled electrodes, in the slice preparation obtained from rats that received chronic knife cuts of the IC at the level of the entopeduncular nucleus. Stimulation of the IC immediately rostral to the STH evoked a fast EPSP followed by a slow EPSP, and IPSPs were largely eliminated in this preparation. The slow EPSP was augmented in MG-free medium and suppressed by 50 μM APV. These results suggest that NMDA receptor mediating slow EPSPs may regulate activities of STH neurons.

Original languageEnglish (US)
Pages (from-to)130-136
Number of pages7
JournalNeuroscience Letters
Volume95
Issue number1-3
DOIs
StatePublished - Dec 19 1988

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Excitatory Postsynaptic Potentials
Neurons
Internal Capsule
Electrodes
Entopeduncular Nucleus
Inhibitory Postsynaptic Potentials
aspartic acid receptor
In Vitro Techniques
Potassium
Injections
Brain

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

An N-methyl-d-aspartate receptor mediated excitatory postsynaptic potential evoked in subthalamic neurons in an in vitro slice preparation of the rat. / Nakanishi, H.; Kita, Hitoshi; Kitai, S. T.

In: Neuroscience Letters, Vol. 95, No. 1-3, 19.12.1988, p. 130-136.

Research output: Contribution to journalArticle

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