An RNA oligonucleotide corresponding to the polypyrimidine region of the rat α1(I) procollagen promoter forms a stable triplex and inhibits transcription

Seth S. Ririe, Ramareddy Guntaka

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10 Citations (Scopus)

Abstract

In this report we demonstrate formation of a triplex structure by an antiparallel RNA oligonucleotide corresponding to the 21 bp polypurine-pyrimidine stretch from -141 to -162 of the rat α1(I) procollagen promoter with a K(d) of 0.1-0.2 μM. The formation of triplexes by the triplex forming oligoribonucleotide (ORN) was also observed under physiological conditions. In vitro transcription run-off experiments showed that triplex formation results in inhibited transcription from the rat α1(I) procollagen gene. Our results demonstrate a novel approach for down-regulation of procollagen gene transcription in vivo.

Original languageEnglish (US)
Pages (from-to)218-221
Number of pages4
JournalBiochemical and Biophysical Research Communications
Volume249
Issue number1
DOIs
StatePublished - Aug 10 1998

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Procollagen
Transcription
Oligonucleotides
Rats
RNA
Genes
Oligoribonucleotides
Down-Regulation
Experiments

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

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AU - Guntaka, Ramareddy

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AB - In this report we demonstrate formation of a triplex structure by an antiparallel RNA oligonucleotide corresponding to the 21 bp polypurine-pyrimidine stretch from -141 to -162 of the rat α1(I) procollagen promoter with a K(d) of 0.1-0.2 μM. The formation of triplexes by the triplex forming oligoribonucleotide (ORN) was also observed under physiological conditions. In vitro transcription run-off experiments showed that triplex formation results in inhibited transcription from the rat α1(I) procollagen gene. Our results demonstrate a novel approach for down-regulation of procollagen gene transcription in vivo.

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