An ultrastructural investigation of tissue-engineered pulp constructs implanted within endodontically treated teeth

Eric L. Gotlieb, Peter E. Murray, Kenneth M. Namerow, Sergio Kuttler, Franklin Garcia-Godoy

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

Background. The authors conducted an ultrastructural scanning electron microscopic (SEM) investigation of tissue-engineered pulp constructs implanted within endodontically treated teeth. Methods. Stem cells from human exfoliated deciduous teeth were seeded on a synthetic open-cell D,D-L,L-polylactie acid scaffold with or without the addition of bone morphogenic protein-2 and transforming growth factor β1 to create pulp tissue constructs. The pulp constructs were implanted into 105 extracted human premolar teeth with a single root canal that had been cleaned and shaped by using rotary instrumentation in a crown-down manner to ISO size no. 35. Results. An ultrastructural examination of the SEM micrographs at ×2,000 magnification revealed cell adherence within all of the pulp constructs, with little difference between the scaffold types or with the addition of growth factors. Conclusions. These results support the proof-of-concept that it is possible to implant tissue-engineered pulp constructs into teeth after cleaning and shaping. Clinical Implications. Future regenerative endodontic treatment may involve the cleaning and shaping of root canals followed by the implantation of vital dental pulp tissue constructs created in the laboratory.

Original languageEnglish (US)
Pages (from-to)457-465
Number of pages9
JournalJournal of the American Dental Association
Volume139
Issue number4
DOIs
StatePublished - Jan 1 2008
Externally publishedYes

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Nonvital Tooth
Dental Pulp Cavity
Tooth
Electrons
Artificial Cells
Dental Pulp
Deciduous Tooth
Endodontics
Bicuspid
Transforming Growth Factors
Crowns
Intercellular Signaling Peptides and Proteins
Stem Cells
Bone and Bones
Acids
Proteins

All Science Journal Classification (ASJC) codes

  • Dentistry(all)

Cite this

An ultrastructural investigation of tissue-engineered pulp constructs implanted within endodontically treated teeth. / Gotlieb, Eric L.; Murray, Peter E.; Namerow, Kenneth M.; Kuttler, Sergio; Garcia-Godoy, Franklin.

In: Journal of the American Dental Association, Vol. 139, No. 4, 01.01.2008, p. 457-465.

Research output: Contribution to journalArticle

Gotlieb, Eric L. ; Murray, Peter E. ; Namerow, Kenneth M. ; Kuttler, Sergio ; Garcia-Godoy, Franklin. / An ultrastructural investigation of tissue-engineered pulp constructs implanted within endodontically treated teeth. In: Journal of the American Dental Association. 2008 ; Vol. 139, No. 4. pp. 457-465.
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