Analysis of a tRNA gene-like sequence (t-element) with TTA at the anticodon position in the mitochondrial DNA of Dictyostelium discoideum

Min Pi, Kiyohiko Angata, Toshimichi Ikemura, Kaichiro Yanagisawa, Yoshimasa Tanaka

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

During the course of mitochondrial DNA sequencing of Dictyostelium discoideum, a sequence with a tRNA-like structure and two genes for tRNAGln(UUG) and tRNATrp(CCA) were found downstream of the gene for large subunit rRNA. The existence of tRNATrp with CCA anticodon supports the finding that UGA codon is not a tryptophan codon in D. discoideum mitochondria. Interestingly, the tRNA gene-like sequence (t-element) has TTA at the anticodon position. Northern blot analysis showed that, in low molecular mass mitochondrial RNA fraction of growth-phase cells and developmental stage cells, a mature transcript of the element could not be detected in the tRNA region on an ureadenatured 15% polyacrylamide gel, although there were several bands in the higher molecular mass region, indicating the actual transcription of the t-element. Southern blot analysis for total and mitochondrial DNA showed that the element exists as a single copy, only in the mitochondrial DNA but not in the nuclear DNA.

Original languageEnglish (US)
Pages (from-to)1-6
Number of pages6
JournalJournal of Plant Research
Volume109
Issue number1093
StatePublished - Mar 1 1996
Externally publishedYes

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Dictyostelium discoideum
mitochondrial DNA
codons
molecular weight
genes
polyacrylamide
Northern blotting
nuclear genome
tryptophan
Southern blotting
mitochondria
sequence analysis
transcription (genetics)
ribosomal RNA
gels
developmental stages
cells
anticodons

All Science Journal Classification (ASJC) codes

  • Plant Science

Cite this

Analysis of a tRNA gene-like sequence (t-element) with TTA at the anticodon position in the mitochondrial DNA of Dictyostelium discoideum. / Pi, Min; Angata, Kiyohiko; Ikemura, Toshimichi; Yanagisawa, Kaichiro; Tanaka, Yoshimasa.

In: Journal of Plant Research, Vol. 109, No. 1093, 01.03.1996, p. 1-6.

Research output: Contribution to journalArticle

Pi, Min ; Angata, Kiyohiko ; Ikemura, Toshimichi ; Yanagisawa, Kaichiro ; Tanaka, Yoshimasa. / Analysis of a tRNA gene-like sequence (t-element) with TTA at the anticodon position in the mitochondrial DNA of Dictyostelium discoideum. In: Journal of Plant Research. 1996 ; Vol. 109, No. 1093. pp. 1-6.
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