Analysis of antibody reactivities in ELISA using protein blots as antigen substrates

S-ELISA

Wai Chun J. Lo, Mark Miller, Ronald C. Montelaro

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

We described here a novel immunoassay procedure, designated strip-ELISA (S-ELISA), which specific antigens are purified by SDS-PAGE, transferred to support membranes, and utilized in situ as substrate in routine ELISA procedures. Using two different lentivirus systems, simian immunodeficiency virus and equine infectious anemia virus, we demonstrate the utility of S-ELISA for screening hybridoma supernatants during production of monoclonal antibodies and for the dissection of polyclonal antibody responses into defined antigen specificities. The data presented here indicates that the S-ELISA combines the sensitivity of conventional ELISA with the specificity afforded by Western blotting procedures, producing a relatively simple and versatile assay for evaluating antibody reactivities.

Original languageEnglish (US)
Pages (from-to)17-25
Number of pages9
JournalJournal of Immunological Methods
Volume128
Issue number1
DOIs
StatePublished - Jan 1 1990
Externally publishedYes

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Enzyme-Linked Immunosorbent Assay
Antigens
Antibodies
Proteins
Equine infectious anemia virus
Simian Immunodeficiency Virus
Lentivirus
Hybridomas
Immunoassay
Antibody Formation
Dissection
Polyacrylamide Gel Electrophoresis
Western Blotting
Monoclonal Antibodies
Membranes

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

Analysis of antibody reactivities in ELISA using protein blots as antigen substrates : S-ELISA. / Lo, Wai Chun J.; Miller, Mark; Montelaro, Ronald C.

In: Journal of Immunological Methods, Vol. 128, No. 1, 01.01.1990, p. 17-25.

Research output: Contribution to journalArticle

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