Analysis of gene expression in the wound repair/regeneration process

Xinmin Li, Subburaman Mohan, Weikuan Gu, David J. Baylink

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

Wound repair/regeneration is a complex process consisting of three stages: inflammation, tissue regrowth, and remodeling, which together involve the action of hundreds of genes. In order to i) identify and analyze the genes that are expressed at the inflammatory stage of repair (i.e., 24 h after injury) and ii) evaluate the molecular basis of fast-wound repair/regeneration in adult mammals, we examined the expression of 8734 sequence-verified genes in response to ear punch in a fast wound-repair/regeneration strain, MRL/MpJ-FasIpr mice, and a slow-wound-repair strain, C57BL/6J mice. Many differentially expressed genes can be assigned to wound-repairing pathways known to be active during the inflammatory phase, whereas others are involved in pathways not previously associated with wound repair. Many genes of unknown function (ESTs) exhibited a more than twofold increase in MRL/MpJ-FasIpr or C57BL/6J mice, suggesting that current understanding of the molecular events at the inflammatory stage of repair is still limited. A comparison of the differential expression profiles between MRL/MpJ-FasIpr and C57BL/6J mice suggests that fastwound-repair in MRL/MpJ-FasIpr mice is mediated by a metabolic shift toward a low inflammatory response and an enhanced tissue repair.

Original languageEnglish (US)
Pages (from-to)52-59
Number of pages8
JournalMammalian Genome
Volume12
Issue number1
DOIs
StatePublished - Feb 19 2001
Externally publishedYes

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Regeneration
Gene Expression
Wounds and Injuries
Inbred C57BL Mouse
Genes
Expressed Sequence Tags
Ear
Mammals
Inflammation

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

Analysis of gene expression in the wound repair/regeneration process. / Li, Xinmin; Mohan, Subburaman; Gu, Weikuan; Baylink, David J.

In: Mammalian Genome, Vol. 12, No. 1, 19.02.2001, p. 52-59.

Research output: Contribution to journalArticle

Li, Xinmin ; Mohan, Subburaman ; Gu, Weikuan ; Baylink, David J. / Analysis of gene expression in the wound repair/regeneration process. In: Mammalian Genome. 2001 ; Vol. 12, No. 1. pp. 52-59.
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