Analysis of Myocilin gene regulatory network using a genetic genomics approach

Hong Lu, Lu Lu, Huai Jin Guan, Hui Chen, Jun Fang Zhang, Nan Hu, Jie Shuai

Research output: Contribution to journalArticle

Abstract

Background: The pathogenesis of primary open angle glaucoma (POAG) and high myopia are very complex. To construct the regulatory network of virulence genes and relevant genes that involved in pathogenicity are helpful for reveal of the pathogenesis. Objective: The aim of this study was to investigate myocilin (Myoc), a gene that contributes to POAG and high myopia in eyes of BXD Recombinant Inbred (BXD RI) mice and construct the regulatory network of Myoc. Methods: The affymetrix microarray system was used to detect the differential expression of Myoc in the eyes of C57BL/6J (B6), DBA/2J (D2) and BXD RI mice. Expression quantitative trait loci (eQTL) mapping was performed to construct the regulatory network of Myoc gene. Results: The average expression level of the Myoc gene in the BXD strains was 10.83, and the gene exhibited expression levels ranging from 8.39 in BXD55 mice toll.43 in B6 mice. The eQTL mapping for the Myoc gene showed a significant likelihood ratio statistic (LRS) of 21.78. The QTL was mapped in chromosome 2, and Myoc was located on chromosome 1, indicating that the Myoc gene was a trans-acting QTL. Olfml2a was identified to be a candidate upstream gene of Myoc by analysis of bioinformatics. Genetic regulatory network analysis demonstrated that a series of genes associated with Myoc probably played roles in the pathogenesis and development of POAG and high myopia. Conclusions: The genetical genomics approach provides a powerful tool for constructing pathways that contribute to complex traits, such as POAG and high myopia.

Original languageEnglish (US)
Pages (from-to)851-854
Number of pages4
JournalZhonghua Shiyan Yanke Zazhi/Chinese Journal of Experimental Ophthalmology
Volume31
Issue number9
DOIs
StatePublished - Sep 1 2013

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Gene Regulatory Networks
Genomics
Myopia
Genes
Quantitative Trait Loci
Virulence
trabecular meshwork-induced glucocorticoid response protein
Chromosomes, Human, Pair 2
Chromosome Mapping
Chromosomes, Human, Pair 1
Computational Biology
Gene Expression
Primary Open Angle Glaucoma

All Science Journal Classification (ASJC) codes

  • Ophthalmology

Cite this

Analysis of Myocilin gene regulatory network using a genetic genomics approach. / Lu, Hong; Lu, Lu; Guan, Huai Jin; Chen, Hui; Zhang, Jun Fang; Hu, Nan; Shuai, Jie.

In: Zhonghua Shiyan Yanke Zazhi/Chinese Journal of Experimental Ophthalmology, Vol. 31, No. 9, 01.09.2013, p. 851-854.

Research output: Contribution to journalArticle

Lu, Hong ; Lu, Lu ; Guan, Huai Jin ; Chen, Hui ; Zhang, Jun Fang ; Hu, Nan ; Shuai, Jie. / Analysis of Myocilin gene regulatory network using a genetic genomics approach. In: Zhonghua Shiyan Yanke Zazhi/Chinese Journal of Experimental Ophthalmology. 2013 ; Vol. 31, No. 9. pp. 851-854.
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abstract = "Background: The pathogenesis of primary open angle glaucoma (POAG) and high myopia are very complex. To construct the regulatory network of virulence genes and relevant genes that involved in pathogenicity are helpful for reveal of the pathogenesis. Objective: The aim of this study was to investigate myocilin (Myoc), a gene that contributes to POAG and high myopia in eyes of BXD Recombinant Inbred (BXD RI) mice and construct the regulatory network of Myoc. Methods: The affymetrix microarray system was used to detect the differential expression of Myoc in the eyes of C57BL/6J (B6), DBA/2J (D2) and BXD RI mice. Expression quantitative trait loci (eQTL) mapping was performed to construct the regulatory network of Myoc gene. Results: The average expression level of the Myoc gene in the BXD strains was 10.83, and the gene exhibited expression levels ranging from 8.39 in BXD55 mice toll.43 in B6 mice. The eQTL mapping for the Myoc gene showed a significant likelihood ratio statistic (LRS) of 21.78. The QTL was mapped in chromosome 2, and Myoc was located on chromosome 1, indicating that the Myoc gene was a trans-acting QTL. Olfml2a was identified to be a candidate upstream gene of Myoc by analysis of bioinformatics. Genetic regulatory network analysis demonstrated that a series of genes associated with Myoc probably played roles in the pathogenesis and development of POAG and high myopia. Conclusions: The genetical genomics approach provides a powerful tool for constructing pathways that contribute to complex traits, such as POAG and high myopia.",
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