Analysis of the Golden Syrian hamster anterior pituitary gland proteome by ion trap mass spectrometry

Charles A. Blake, David G. Kakhniashvili, Steven Goodman

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

We utilized mass spectrometry (MS) and bioinformatics to investigate the proteome of the anterior pituitary gland (AP). Subcellular fractions of APs from 2-month-old male Golden Syrian hamsters were prepared for protein denaturation, treatment with trypsin and analyses utilizing micro liquid chromatography MS/MS and the database search software SEQUEST. In the nuclear, non-nuclear 100,000 x g and cytosolic fractions we identified 76, 52 and 52 different proteins, respectively. A total of 145 distinct proteins were detected. We identified growth hormone, prolactin, pro-opiomelanocortin, the α-subunit for the glycoprotein hormones, luteinizing hormone-β and follicle-stimulating hormone-β. Groups of other identified proteins included hormone processing, secretion granule associated, non-hormonal endoplasmic reticulum associated, calcium binding, protein kinase C associated histone and non-histone chromosomal material, other RNA-binding, splicing factors, heterogeneous nuclear ribonucleoproteins, helicases, lamins, microfilament associated, microtubule associated, adenosine triphosphate and guanosine diphosphate associated, keratins, lysosomal, ribosomal, enzymes in glycolysis and the tricarboxylic and pentose phosphate paths, glutathione associated, transmethylation, catabolic and unknown protein products as well as blood hemoglobins. Proteins previously not reported in the AP, such as fertility protein SP22, were identified. The proteins identified in the present study form a foundation for defining the proteome in normal adult male AP.

Original languageEnglish (US)
Pages (from-to)355-367
Number of pages13
JournalNeuroendocrinology
Volume80
Issue number6
DOIs
StatePublished - Dec 1 2004
Externally publishedYes

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Anterior Pituitary Gland
Mesocricetus
Proteome
Mass Spectrometry
Ions
Proteins
Hormones
Lamins
Heterogeneous-Nuclear Ribonucleoproteins
Pentoses
Protein Denaturation
Pro-Opiomelanocortin
Calcium-Binding Proteins
Subcellular Fractions
Guanosine
Diphosphates
Follicle Stimulating Hormone
Glycolysis
Luteinizing Hormone
Keratins

All Science Journal Classification (ASJC) codes

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology
  • Endocrine and Autonomic Systems
  • Cellular and Molecular Neuroscience

Cite this

Analysis of the Golden Syrian hamster anterior pituitary gland proteome by ion trap mass spectrometry. / Blake, Charles A.; Kakhniashvili, David G.; Goodman, Steven.

In: Neuroendocrinology, Vol. 80, No. 6, 01.12.2004, p. 355-367.

Research output: Contribution to journalArticle

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