Analysis of the inducer‐responsive CAR1 upstream activation sequence (UASI) and the factors required for its operation

Ladislau Z. Kovari, Heui‐Dong ‐D Park, Iulia A. Kovari, Terrance Cooper, Marlene Fourie, Hendrik J.J. van Vuuren

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Induced production of arginase (CAR1) enzyme activity and steady‐state CAR1 mRNA in Saccharomyces cerevisiae requires wild‐type ARG80/ARGRI and ARG81/ARGRII gene products. We demonstrate here that these gene products, along with that of the MCM1 gene, are required for the inducer‐dependent UASI‐A, UASI‐B and UASI‐C elements to function but they are not required for operation of inducer‐independent CAR1 UASC1 or UASC2M. Through the use of single and multiple point mutations, the CAR1 UASI‐B and UASI‐C elements were demonstrated to be at least 23 bp in length. Moreover, simultaneous mutation of both ends of an elements gave stronger phenotypes than mutations at either end. The center of the element was more sensitive to mutation than were the ends.

Original languageEnglish (US)
Pages (from-to)835-845
Number of pages11
JournalYeast
Volume9
Issue number8
DOIs
StatePublished - Jan 1 1993

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Genes
Chemical activation
Mutation
Arginase
Enzyme activity
Point Mutation
Yeast
Saccharomyces cerevisiae
Phenotype
Messenger RNA
Enzymes

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Genetics

Cite this

Analysis of the inducer‐responsive CAR1 upstream activation sequence (UASI) and the factors required for its operation. / Kovari, Ladislau Z.; Park, Heui‐Dong ‐D; Kovari, Iulia A.; Cooper, Terrance; Fourie, Marlene; van Vuuren, Hendrik J.J.

In: Yeast, Vol. 9, No. 8, 01.01.1993, p. 835-845.

Research output: Contribution to journalArticle

Kovari, Ladislau Z. ; Park, Heui‐Dong ‐D ; Kovari, Iulia A. ; Cooper, Terrance ; Fourie, Marlene ; van Vuuren, Hendrik J.J. / Analysis of the inducer‐responsive CAR1 upstream activation sequence (UASI) and the factors required for its operation. In: Yeast. 1993 ; Vol. 9, No. 8. pp. 835-845.
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